蛋白激酶C在心肌细胞肥大中的作用

摘要

Objective:The present study was to investigate the role ofprotein kinase C(PKC)in the hypertrophy of cardimyocytes.Methods:The measurement of cell surface area and [3H]-Leucine incorporation judged the hypertrophy of cardiaomycocyte in cultured neonatal rat myocardal cells.Results:The results showed that adrenaline could significantly increase cell surface area and promote [3H]-Leucine incorporation.PKC inhibitor Calphostin C could markedly block effects of adrenaline increasing cell surface area and promoting [3H]-Leucine incorporation.Conclusions:These results suggest that the hypertrophy of cardiomyocytes induced by adrenaline in cultured neonatal rat myocardal cells is relation PKC.%目的：要探讨蛋白激酶C(PKC)在心肌细胞肥大中的作用机制。方法：在培养新生大鼠心肌细胞上，通过测量心肌细胞表面积和[3H]-Leu的掺入量来判断心肌细胞肥大。结果：肾上腺素能明显增加心肌细胞表现积和[3H]-Leu的掺入量，PKC抑制剂(CalphostinC)可抑制肾上腺素诱导的心肌细胞表面积和[3H]-Leu掺入量增加。结论：PKC在肾上腺素诱导的心肌细胞肥大反应中起重要作用。