目的 探讨唾液是否为乙型肝炎病毒传播的途径之一，为乙型肝炎病毒经唾液传播提供临床依据。方法 采用时间分辨荧光免疫分析法检测免疫学指标，实时荧光定量PCR法检测乙肝患者血清、唾液HBVDNA含量。结果 119例乙肝患者血清、唾液HBV DNA的阳性率分别为91.6％（109例）、71.4％ (85例)。乙肝患者唾液HBV DNA的阳性率与血清HBV DNA的阳性率之间差异有统计学意义(P＜0.01)，乙肝患者唾液HBV DNA含量与血清HHBV DNA含量呈显著正相关（r=0.83）。结论 乙型肝炎病毒可通过唾液传播。%Objective To investigate whether saliva is one of the important pathways for the transmission of hepatitis B virus (HBV), and to provide clinical evidences the spreading of HBV through saliva. Methods Immuno-logical indicators were determined with time-resolved fluoroimmunoassay (TR-FIA), and the contents of HBV DNA in serum and saliva were determined with real-time polymerase chain reaction (FQ-RT-PCR). Results In 119 cases of hepatitis B, the positive rate of HBV DNA in serum and saliva was 91.6% (n=109) and 71.4% (n=85), respectively, which showed a statistically significant difference (P<0.01). A positive correlation was found between the content of HBV DNA in serum and that in saliva (r=0.83). Conclusion This study provides clinical evidences for the transmission of HBV through saliva.
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