饲喂高浓度创伤弧菌对小鼠消化道的损伤研究

摘要

目的 探讨高浓度创伤弧菌(Vibrio vulnificus,Vv)经食道感染小鼠的致病特点.方法 22只昆明小鼠,实验组16只,均强制性喂食Vv菌液(4.2×1012 cfu/ml) 0.6 ml/只；对照组6只,喂食灭菌液体培养基(Marine broth 2216) 0.6 ml/只.观察小鼠一般情况,分别于实验后12h及48h采血并处死8只实验组小鼠和3只对照组小鼠,进行血液的Vv分离培养并取其消化道及重要脏器组织进行病理学观察.结果 48h组8只小鼠,有4只血培养结果为阳性；Vv经食道感染小鼠后导致其出现腹泻等消化道症状,病理学结果显示实验组小鼠食道及胃黏膜上皮部分缺损,小肠绒毛明显水肿,结肠肠壁平滑肌溶解断裂,消化道黏膜、黏膜下、肌层均可见不同程度的中性粒细胞浸润；小鼠主要脏器也出现不同程度的损伤,以肺和肾脏的广泛出血性损伤为主.结论 (1)高浓度Vv经食道进入小鼠胃组织后能部分存活,并进入肠道繁殖,从而引起小鼠腹泻症状,损伤小鼠消化道组织,并可入血引发败血症,导致小鼠肺、肾等多脏器的和损伤,造成小鼠死亡.(2)Vv可经消化道感染,所致炎症性病变特点为蜂窝织性炎.%Objective To study the characteristics of infections through the alimentary canal with Vibrio vulnificus (Vv) in mice. Methods Twenty-two KM mice (SPF) were randomly divided into two groups, the study group (n=16) and the control group (n=6). The animal model of Vibrio vulnificus infections through the alimentary canal was established by feeding Vv inoculum (4.2× 1012 cfu/ml, strain 1.175 8) of 0.6 ml to each mouse in the study group. And the mouse in the control group was fed with sterile culture medium (Marine broth 2216). Eight mice in the study group and three mice in the control group were killed 12 h and 48 h after treatment The Vv from the blood were cultured, and the pathological changes of the alimentary canal and other organs were observed under microscope. Results Of the eight mice killed 48 h after treatment, four showed positive blood cultures. In the study group, The gastric and esophagus mucosa was damnified with degeneration and necrosis of the epidermic cells. The edema of small intestinal villous was also observed. The neutrophil infiltration was observed in each layer of digestive tract. And different degrees of injury were also observed in the main viscera of the mice in study group, which is the most severe in lung and kidney. The pathological changes of lung and kidney were characterized by hemorrhagia, degeneration and necrosis of the cells. Conclusion The injury of digestive tract could be induced by Vv in high concentration. Vv reproduction in digestive tract leads to primary septicemia, resulting in the death of the mice finally. Vv infection in digestive tract was characterized by Phlegmonous inflammation.