目的 观察创伤弧菌菌体抗原及溶细胞毒素蛋白抗原对Vv感染小鼠的免疫保护作用,以期为Vv防治提供实验数据.方法 制作创伤弧菌菌体抗原及溶细胞毒素蛋白抗原,免疫BALB/c小鼠后观察免疫状态改变及其对Vv感染小鼠的免疫保护效应.结果 免疫后小鼠实验组CD19+B淋巴细胞百分比高于对照组,并产生相应特异性抗体,效价最高达1∶25600,创伤弧菌攻击实验实验组小鼠存活率为100％,显著高于对照组的13.33％.结论 创伤弧菌菌体抗原及溶细胞毒素蛋白抗原主动免疫能产生特异性抗体,能够有效对抗创伤弧菌感染,并明显提高小鼠的存活率.%Objective To investigate whether Vibrio vulnificus bacterial protein antigen and the cytolytic toxin antigen can induce the effective immune protection against Vibrio vulnificus infection.Methods BALB/c mice were immunized with bacterial cytolytic toxin antigen protein antigen of Vibrio vulnificus to evaluate its ability to stimulate immune response.The protective efficacy of immunized mice was evaluated by active immunization and intraperitoneal challenge with V.vulnificus in mice.Results The immunized mice produced higher percentage of CD19+ B lymphocytes and high level specific antibodies (titers up to 1∶25600).All immunized mice survived from lethal challenge with V.vulnificus,while only 13.33％ of mice in control group survived at the end of challenged experiment.Conclusions The bacterial protein antigen and cytolytic toxin antigen of Vibrio vulnificus are capable of inducing specific antibody response in mice to confer effective protection against lethal challenge with V.vulnificus.
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