三维适形调强放疗治疗新疆骨棘球蚴病大鼠后T淋巴细胞亚群的变化及意义

摘要

目的：通过观察三维适形调强放疗(Intensity modulated radiation therapy，IMRT)对新疆大鼠继发性骨棘球蚴进行治疗后大鼠T淋巴细胞亚群的影响，探讨IMRT对骨包虫的杀灭机制是否与射线激发宿主对骨包虫有效的免疫反应有关。方法选取股骨继发性棘球蚴病大鼠50只，随机分为IMRT组及空白对照组各25只，以IMRT方式分别对IMRT组25只大鼠左侧股骨棘球蚴病区给予40 Gy剂量的放疗，于放疗1周后所有大鼠活杀取材，取脾制备细胞悬液,用流式细胞术(FCM)检测CD4+/CD8+的值。结果 IMRT作用1周后，流式细胞仪检测结果提示，IMRT组大鼠脾淋巴细胞CD4+/CD8+的值(2.96±1.90)明显高于空白对照组(1.65±1.31)，差异具有统计学意义(t=0.544，P<0.05)。结论 IMRT可促进新疆大鼠继发性骨棘球蚴的凋亡，其作用机制可能是与射线激发宿主对骨包虫有效的免疫反应有关。%Objective To observe the change and significance of T lymphocyte subsets in rats of Xinji-ang with echinococcosis after intensity modulated radiation therapy (IMRT), and explore the possible mechanism. Methods Fifty cases of rats with secondary echinococcosis at femurs were selected and randomized into two groups equally. 25 rats in IMRT group were given IMRT at the left femur, where the echinococcosis located,with a total irra-diation doses of 40 Gy. And one week after radiation, the spleen cells were collected from all rats. The value of CD4+/CD8+ was detected by flow cytometry. Results One week after IMRT, the value of CD4+/CD8+ in the IMPT group (2.96±1.90) was significantly higher than that in the blank control group (1.65±1.31). Conclusion IMRT contributes to the therapy of secondary echinococcosis,with a underlying mechanism of activation in effective parasite-host im-mune response which is initiated by radiation.