趋化因子CXCL10启动子区-201G/A多态性与慢性乙型肝炎易感相关性研究

摘要

Objective To investigate the association between the single nucleotide polymorphism (SNP) in-201 loci of chemotactic factor CXCL10 gene promoter region and the susceptibility to chronic hepatitis B (CHB). Methods One hundred and forty-two patients with chronic hepatitis B (CHB group) and 150 healthy controls (HC group) were included in our research. Genotypes of -201 loci in the chemotactic factor CXCL10 gene promoter were examined by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (T-Bil), gamma glutamyl transpeptidase (GGT), albumin (ALB), hepatitis B e antigen (HBeAg), hepatitis B virus (HBV-DNA) and the HBV-DNA viral load, hepatitis B virus large protein (HBV-LP) and hepatitis B virus S1 antigen (HBV-PreS1) were de-tected. Results Compared with HC group, the frequency distribution of the GA&AA genotype of CXCL10-201A/G was significantly increased in CHB group (P<0.05), as well as the frequency distribution of the A-allele. Moreover, the A-allele of-201 loci may be associated with the susceptibility to CHB virus (OR=1.940, OR95%CI:1.139~3.305). CXCL10-201A/G SNP was not associated with the positive rate of ALT, AST, HbeAg, HBV-DNA, HBV-LP, HBV-PreS1 (P>0.05). When HBV-DNA viral load in the CHB group was in the range of 105~106 copies/ml, the fre-quency distribution of A-allele and G-allele was statistically significant (χ 2=3.958, P=0.047). Conclusion SNP in-201 loci of CXCL10 gene promoter region is associated with susceptibility to chronic hepatitis B, and A-allele may be the susceptible factors of HBV infection.%目的：研究趋化因子CXCL10启动子区-201G/A位点单核苷酸多态性(SNP)与汉族人群慢性乙型肝炎(CHB)的易感相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对142例慢性乙型肝炎患者(CHB组)和150名健康者(HC组)的CXCL10启动子区-201G/A SNP进行基因分型，以血清丙氨酸氨基转氨酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(T-Bil)、γ-谷氨酰转肽酶(GGT)、白蛋白(ALB)、乙型肝炎E抗原(HBeAg)、乙型肝炎病毒(HBV-DNA)及其病毒载量、乙肝病毒大蛋白(HBV-LP)、乙型肝炎病毒前s1抗原(HBV-PreS1)作为检测指标。结果与HC组比较，CHB组的CXCL10-201位点GA＆AA基因型及A等位基因分布频率显著增加，组间差异具有统计学意义(P<0.05)，且等位基因A可增加CHB易感风险(OR=1.940，OR95%CI：1.139~3.305)。CXCL10-201G/A多态性与ALT、AST、HBeAg、HBV-DNA、HBV-LP、HBV-PreS1阳性率无相关性(P>0.05)；CHB组病毒载量在105~106取值范围时，等位基因A与G分布频率差异具有统计学意义(χ2=3.958，P=0.047)。结论 CXCL10启动子区-201G/A位点多态性与CHB患病风险有关联，且等位基因A可能是HBV易感基因。