大鼠糖尿病性勃起功能障碍模型的制备

摘要

Objective To investigate the ideal method for establishing diabetic rats model with erectile dys-function (ED). Methods Ninety male SD rats were randomly divided into 3 groups (groupⅠ,Ⅱ,Ⅲ), each with 30 rats. Each group were further divided into control (CN) subgroup and diabetes mellitus (DM) subgroup equally, with 15 rats in each subgroup. SD rats were injected intraperitoneally with 65 mg/kg streptozotocin (STZ) to make experi-mental models of DM (DM subgroup), and equal volume of citrate buffer was injected in the CN subgroup. Injected with apomorphine, penile erection of the rat models were observed 8 (groupⅠ), 12 (groupⅡ), and 16 weeks (groupⅢ) after establishment of models. Results The times of penis erection in DM subgroups of groupⅠ,Ⅱ,Ⅲwere (1.0±0), (1.0±0), (1.0±0), respectively, and the rates of penis erection were only 33.3%, 21.4%, 14.3%, with the inci-dences of ED of 66.7%, 78.6%, 85.7%. The times of penis erection in CN subgroups of groupⅠ,Ⅱ,Ⅲwere (2.2±0.8), (2.3±0.8), (2.0±0.7), respectively, and the rates of penis erection were all 100%, with the incidences of ED of 0. There was statistically significant differences in the rates of penis erection between the DM subgroup and the CN subgroup (P<0.01). Conclusion Intraperitoneal injection with STZ (65 mg/kg) is a safe method for establishment of diabetic rat model. Subcutaneous injection with apomorphine (80μg/kg) is effective to screen diabetic rat model with erectile dys-function.%目的：探讨理想糖尿病性勃起功能障碍(ED)大鼠模型的制备方法。方法90只SD大鼠，随机分为Ⅰ、Ⅱ、Ⅲ三组各30只，每组再分为正常组(CN)和糖尿病组(DM)，每组各15只，DM组腹腔注射链脲佐菌素(STZ，65 mg/kg)诱导糖尿病大鼠模型，CN组腹腔注射等量体积的柠檬酸盐缓冲液。成模8周、12周及16周注射阿朴吗啡(APO，80µg/kg)观察大鼠阴茎勃起情况，制备糖尿病性ED大鼠模型。结果Ⅰ、Ⅱ、Ⅲ三组中的糖尿病大鼠在8、12、16周注射阿朴吗啡后勃起次数分别为(1.0±0)次、(1.0±0)次、(1.0±0)次，勃起率分别为33.3%、21.4%、14.3%，ED发生率分别为66.7%、78.6%、85.7%。Ⅰ、Ⅱ、Ⅲ三组中的正常大鼠勃起次数分别为(2.2±0.8)次、(2.3±0.8)次、(2.0±0.7)次，勃起率均为100%，ED发生率为0。各组间勃起率比较差异均有显著统计学意义(P<0.01)。结论采用STZ 65 mg/kg腹腔注射建立糖尿病大鼠模型方法安全有效，颈部皮下注射APO 80μg/kg筛选糖尿病ED大鼠可行可靠。