Objective To investigate the resistance of bacteria isolated from clinic in our hospital, and to pro-vide reference for the rational use of antibiotics in clinical practice. Methods Antimicrobial susceptibility testing was performed with Kirby-Bauer method. All the data were analyzed by WHONET 5.5 and SPSS18.0 softwares ac-cording to the breakpoints of CLSI 2011. Results A total of 4 176 clinical strains were isolated during 2012, of which Gram positive cocci and Gram negative bacilli accounted for 26.2% and 73.8%, respectively. The resistance rates of S. aureus (MRSA) and coagulase negative Staphylococcus (MRCNS) to methicillin were 51.7%and 71.7%, re-spectively. In Enterococcus spp, the resistance rates of E. faecalis strains to ampicillin, macrodantin and fosfomycin were much lower than those of E. faecium. There were no Staphylococcal and Enterococcus spp. strains resistant to van-comycin, teicoplanin or linezolid. The prevalences of ESBLs producing strains were 65.1% in E. coli and 31.3% in Klebsiella spp. ESBLs-prodcing Enterobacteriaceae strains were much more resistant than non-ESBLs-producing strains in respect of antibiotic resistance rate. The Enterobacteriaceae strains were still highly susceptible to car-bapenems, and the resistance rates of these strains to carbapenems were less than 2.2%. The resistance rates of Acinetobacter baumannii in our hospital to imipenem, meropenem, cefperazone-sulbactam and minocycline were 88.6%, 84.2%, 59.6% and 42.6%, respectively. The detection rate of multidrug-resistant Acinetobacter baumannii reached up to 40.5%. Less than 20.0%of Pseudomonas aeruginosa isolates were resistant to third, fourth cephalo-sporin and carbapenems. Conclusion The detection rates of MRSA, MRCNS, multidrug-resistant Acinetobacter baumannii and ESBLs-prodcing Enterobacteriaceae strains in our hospital were surprisingly high. The surveillance of antibiotic resistance and the management of antibacterials grading system should be strengthened, so as to promote the rational use of antimicrobials.%目的：了解我院临床分离株菌对常用抗菌药物的耐药性，为临床合理使用抗菌药物提供依据。方法采用纸片扩散法进行药敏试验，以CLSI2011年标准判断分离株的敏感性，用WHONET 5.5及SPSS18.0软件分析数据。结果2012年我院共收集临床分离菌株4176株，其中革兰氏阳性菌株1094，占26.2%，革兰氏阴性菌3082株，占73.8%。金黄色葡萄球菌和凝固酶阴性葡萄球菌对甲氧西林的耐药率分别为51.7%和71.7%。肠球菌属中粪肠球菌对氨苄西林、呋喃妥因、磷霉素的耐药率要明显低于屎肠球菌。未发现对万古霉素、替考拉宁、利奈唑胺耐药的葡萄球菌属及肠球菌属。我院大肠埃希菌和肺炎克雷伯菌产ESBLs的比例分别为65.1%和31.3%。肠杆菌科细菌中产ESBLs株对所检测的抗菌药物的耐药率均明显高于非产ESBLs菌株。肠杆菌科细菌对碳青酶烯类药物敏感率极高，耐药率不超过2.2%。我院鲍曼不动杆菌对亚胺培南、美罗培南、头孢哌酮-舒巴坦、米诺环素的耐药率分别为88.6%、84.2%、59.6%、42.6%，其中多重耐药鲍曼不动杆菌的检出率达40.5%。分离的铜绿假单胞菌对三、四代头孢菌素，碳青霉烯类等抗菌药物的耐药率均不超过20.0%。结论我院耐甲氧西林的金葡菌(MRSA)、凝固酶阴性葡萄球菌(MRCNS)、多重耐药鲍曼不动杆菌以及肠杆菌科细菌产ESBLs的检出率极高，应加强细菌耐药性监测和抗菌药物分级管理，合理使用抗菌药物。
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