胃癌组织中VGLL4、YAP蛋白表达与胃癌患者预后的关系

摘要

目的 检测胃癌组织及癌旁组织中VGLL4、YAP蛋白的表达情况,探讨其在胃癌诊治中的临床意义.方法 采用免疫组织化学SP法检测46例胃癌组织及癌旁组织中VGLL4、YAP蛋白的表达情况,分析其表达与胃癌患者预后的相关性.结果 胃癌组织中VGLL4、YAP蛋白的阳性表达率分别为39.1％(18/46),76.1％(35/46),癌旁组织中的阳性表达率分别为91.3％(42/46)、10.9％(5/46),差异有统计学意义(P<0.05).VGLL4和YAP蛋白的表达均与胃癌组织学分级、临床分期、淋巴结转移、侵袭性具有相关性(P<0.05),且与患者预后相关.YAP阳性表达高水平胃癌患者术后中位生存时间[(19.000±0.557)个月]明显短于YAP阳性表达低水平胃癌患者[(36.000±2.942)个月],VGLL4阳性表达低水平胃癌患者术后中位生存时间[(18.000±1.503)个月]明显短于VGLL4阳性表达高水平胃癌患者[(26.000±2.863)个月],YAP和VGLL4表达水平均与胃癌患者预后明显相关,差异有统计学意义(P<0.05).结论 VGLL4在胃癌组织中较癌旁组织表达下降,且与肿瘤发展和恶性程度呈负相关,是一种潜在的抑癌蛋白.YAP在胃癌组织中较癌旁组织表达增高,与肿瘤发展和恶性程度呈正相关,且两者均与患者预后相关.%Objective To detect the expression of vestigial-like family member 4 (VGLL4) and yes-associated protein (YAP) in gastric cancer tissue and paracancerous tissue, and to analyze its clinical significance in the diagnosis and treatment of gastric cancer. Methods The expression of VGLL4 and YAP protein in 46 cases of gastric carcinoma and paracancerous tissue were detected by SP immunohistochemical method, and the correlation between the expression and prognosis of patients was analyzed. Results The positive expression rates of VGLL4 and YAP protein were respec-tively 39.1％(18/46) and 76.1％(35/46) in gastric cancer tissues versus 91.3％(42/46) and 10.9％(5/46) in paracancer-ous tissues (P<0.05). The expression of VGLL4 and YAP protein were correlated with the histological grade, clinical stage, lymph node metastasis and invasion (P<0.05), and correlated with the prognosis. The median survival of patients with high level of YAP positive expression was (19.000 ± 0.557) months, which was significantly shorter than (36.000 ± 2.942) months of the patients with low levels of YAP positive expression (P<0.05);the median survival of patients with low level of VGLL4 positive expression was (18.000 ± 1.503) months, which was significantly shorter than (26.000 ± 2.863) months of the patients with high levels of VGLL4 positive expression (P<0.05). Conclusion Compared with the adjacent tissue, the expression of VGLL4 in gastric cancer tissue shows a significant downward trend, and the degree of tumor devel-opment and malignancy is negatively correlated with the expression of VGLL4, as a potential anti-cancer protein. YAP is a proto-oncogene, which is highly expressed in gastric cancer tissues, and positively correlated with tumor progression and ma-lignancy. The expression of VGLL4 and YAP protein is correlated with the prognosis of gastric cancer.