Tuberculosis(TB)is a major global infectious disease.Rapid and accurate diagnostic methods are vi-tal in the prevention and control of tuberculosis.Early detection of TB can maximize the speed control of its spread and epidemic.At present,the routine diagnostic method is bacteriological detection,but the sensitivity of that test is low.En-zyme linked immunosorbent assay(ELISA)is a widely used serological diagnostic method.It has the characteristics of convenient,rapid,and simple.In this paper,the principle of ELISA,the selection of specific antigen and the clinical val-ue of the method are reviewed.%结核病是一个全球性的重大传染病.快速、准确的诊断方法在结核病的综合防控策略中极其重要,早期发现结核病可最大限度控制其蔓延和流行的速度.目前常规诊断方法为细菌学检测,但细菌学检测方法的诊断效能较低.酶联免疫吸附试验是一种血清学诊断方法,具有方便、快速、简单的特点.本文就ELISA法的原理、特异抗原的选择、临床应用价值进行了综述.
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