抗马铃薯Y病毒的ihpRNA高效茎长度的选择

摘要

为获得干涉基因片段的最小有效干涉长度,利用Gateway技术,构建出4个具有不同马铃薯Y病毒(PVY)外壳蛋白基因片段的RNAi载体(Phe-Y246、Phe-Y199、Phe-Y143和Phe-Y103),瞬时表达检测这4个干涉载体的干涉效果.结果表明:20株浸润有Phe-Y143载体的抗病毒植株比例达100%,Phe-Y246、Phe-Y199和Phe-Y103载体浸润植株的抗病毒比例分别为85%、90%和80%.表明,PVY外壳蛋白基因的高效抗性片段的茎长度为104～143 bp.%To obtain the minimum length of effective interference on the research of the crops resisting multi-viruses,four expressing vectors harboring different part of coat protein gene in potato virus Y were constructed using gateway technology, which named Phe-Y246、 Phe-Y199、 Phe-Y143 and Phe-Y103 respectively. The result of transient expression showed that the ratio of antivirial crops is close to 100％ among 20 strains of nicotiana benthamiana infiltrated with Phe-Y143 and the others(Phe-Y246, Phe- Y199,Phe-Y103 ) of that are 85 ％ 、90 ％ and 80 ％ respectively. It is concluded that the minimum length of effective interference is between 103bp and 143bp.