黑武士大叶醉鱼草茎段愈伤组织的诱导及植株再生

摘要

The isolated culture and tube seedling regeneration of B. davidii explants were studied to establish a rapid propagation and genetic breeding system of Heiwushi B. davidii. The results showed that the optimum culture media for calli induction and differentiation were MS + 2. 0 mg/L 2,4-D+0. 2 mg/L 6-BA and MS+2. 0 mg/L TDZ+0. 2 mg/L NAA with the induction rate and differentiation rate of 85% and 80% separately. The average single bud number was 13. The optimum rooting medium was 1/2 MS+0. 5 mg/L IBA. The rooting rate and survival rate were 78. 7% and 80% respectively.%为了建立黑武士大叶醉鱼草的快速繁殖与遗传育种体系,以大叶醉鱼草的茎段为外植体,研究其离体培养及试管苗再生途径.结果表明:愈伤组织诱导的最佳培养基为MS+2.0 mg/L2,4-D+0.2mg/L 6-BA,诱导率为85％；愈伤组织分化的最佳培养基为MS+2.0 mg/L TDZ+0.2 mg/L NAA,分化率为80％,平均单芽数高于13个；最佳生根培养基为1/2MS+0.5 mg/L IBA,生根率为78.7％,移栽成活率为80％.