采用高效液相色谱技术分析烟草体内的维生素B6化合物

摘要

Vitamin B6(VB6)is the general term for a kind of chemical compounds . VB6 exists in several forms,and has been linked to stress responses in plants. Until now no reports about the distribution of B6 vitamers in tobacco plants have been observed. In our experiment,the determination of VB6 vitamers in tobacco plants was described by using HPLC with fluorescence detector. The results indicated that, the contents of VB6 in leaves, tender stem and roots were 2. 9,1. 7 and 3. 0 μg/g fresh weight,respectively. Leaves of tobacco plants grown on MS basal media exhibited a high content of 3. 9 μg/g fresh weight. The constituent ratio of B6 vitamers were as follows: pyridoxamine 5'-phosphate(PMP) 7% , pyridoxamine(PM) 14%, pyridoxal 5'-phosphate(PLP) 19%, pyridoxal(PL) 29% and pyridoxine(PN) 30%. During the determination period of three weeks,the contents of PLP and PL decreased,and that of PN increased. The amount of VB6 was relatively constant. Our results would be favorable for further study on the metabolic mechanism and special physiological mechanism in tobacco plants.%维生素B6 (VB6)是一类化合物的总称.近年来研究发现VB6在植物体内发挥抗逆作用.烟草作为模式植物其体内VB6的存在形态还未见报道.本研究采用高效液相色谱结合荧光检测技术对烟草体内VB6的存在形态进行了分析.结果表明:土壤栽培烟草叶、茎和根中VB6的含量依次为2.9、1.7、3.0 μg/g鲜重;组培烟草叶片的VB6含量为3.9 μg/g鲜重,构成比为磷酸吡哆胺(PMP)7％、吡哆胺(PM)14％、磷酸吡哆醛(PLP)19％、吡哆醛(PL)29％、吡哆醇(PN)30％;组培烟草在连续3周的检测过程中,PLP和PL含量下降、PN含量上升,VB6总量保持相对稳定.研究结果有助于以烟草为材料,进一步开展植物体内VB6代谢机制和特殊生理机制的研究.