A method was established for simultaneous determination of vitamin B2, vitamin B3 and vitamin B6 in toothpaste. The samples were dispersed with water and impurities were removed with acetone, and then the samples were separated with a T3 chromatographic column and determined by HPLC. The selection and dosage of the precipitating agent were optimized in this work. The results showed that, under optimized conditions, the linear was good, and R2>0.999, the LOD of the method (S/N=3) was between 0.60 and 1.20 mg/kg. The average recoveries were from 95.0% to 103.7%, and the relative standard deviations were in a range of 1.67% ~ 6.96% when three levels of standard were spiked into toothpaste samples. The method is simple, accurate, sensitive and rapid, so it is suitable for quantitative analysis of Vitamin B2, Vitamin B3 and Vitamin B6 in toothpaste.%建立了同时测定牙膏中维生素B2、维生素B3和维生素B6的检测方法.样品经水分散溶解后,用丙酮沉淀杂质,采用Waters T3色谱柱分离,HPLC测定,实验优化了沉淀剂的选择及用量等条件.结果表明,待测维生素在各自浓度范围内线性良好,相关系数均大于0.999,方法检出限在0.60~1.20 mg/kg之间,在高中低三个添加水平的平均回收率为95.0%~103.7%,相对标准偏差为1.67%~6.96%.该方法具有前处理简单、定量准确、灵敏快速的特点,适用于牙膏中维生素B2、维生素B3和维生素B6的定量分析检测.
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