雪松不同部位中原花青素的含量测定及其清除 DPPH 自由基的活性

摘要

Cedrus deodara (Pinaceae family),which consists of C .deodara ,C .libani ,C .brevifolia and C .atlan-tica ,is an evergreen tree growing extensively on the slopes of the Himalayas.Its leaves was a kind of chinese medi-cine for a long history.Its main chemical constituents include flavonoids,phenylpropanoids,organicacid,triterpenes, steroids,polysaccharides leaf glue and so on.The major pharmacological activities of pine needles of C .deodara in-clude antitumor,antioxidation,improving memory,antibacterial and antivirus,ect.In order to determine proantho-cyanidin content and DPPH radical scavenging activity of different parts from C .deodara .In this study,Fe3+ ion was used for catalysator,n-BuOH∶HCl (95∶5)was chromogenic agent and the detection wave-lengths were at 550 nm. And the content of proanthocyanidin was determined in different parts from C .deodara by ultraviolet-visible spectro-photometer.And antioxidant activity of extract from different parts of C .deodara were investigated by scavenging 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH).The results showed that proanthocyanidins content in different parts of C .deodara was pine needles(12.93%),inflorescence (12.93%),bark(12.00%),wood (6.41%),tender branchlets(3.37%).The extract from different parts of C .deodara had good antioxidant activity.In a certain concen-tration scope,there was a strong positive correlation between the scavenging activity and the concentrations.The or-der of IC50 was tender branchlets > pine needles > wood> bark > inflorescence.Except for branchlets,their DPPH scavenging activities were around 90% while the concentration of the extract from different parts was 0.8 mg.mL-1 . The results indicated that there were higher proanthocyanidins content and antioxidant activities in different parts of C .deodara ,which provide the reference on comprehensive development and utilization of C .deodara .%雪松(Cedrus deodara )是松科(Pianaceae)雪松属(Cedrus )树种的泛称，为广泛分布于喜马拉雅山脉的常绿树种，包括雪松(C ．deodara )、黎巴嫩雪松(C ．libani )、短叶雪松(C ．brevifolia )和北非雪松(C ．atlan-tica )等。雪松的针叶药用历史悠久，主要化学成分为黄酮类、苯丙素类、有机酸类、三萜类、甾体类、多糖及针叶胶等，具有抗肿瘤、抗氧化、改善记忆、抗菌及抗病毒等多种功效。为了测定雪松不同部位中原花青素的含量，并考察其清除 DPPH 自由基活性的能力，该研究以 Fe3＋离子为催化剂，用正丁醇－盐酸法分别测定雪松松针、花序、小枝、木质部、树皮中原花青素的含量，并对不同部位提取液清除 DPPH 自由基的能力进行测定。结果表明：松针、花序、木质部、树皮、小枝中原花青素的含量分别为12．93％、12．93％、6．41％、12．00％、3．37％，在质量浓度(以生药计)为0．8 mg.mL-1时，其对 DPPH 自由基的清除率均达到或接近90％。该研究结果提示雪松各个部位的原花青素含量丰富，其提取液的体外抗氧化活性很高，这为雪松的进一步开发利用提供了很好的参考价值。