SPME-GC/MS 联用分析六堡茶茶花香气成分

摘要

The tea plant flowers of indigenous species in Liubao, Guangxi were characteristic with long florescence, high yield and rich fragrance, but its chemical composition has not been reported for the development and utilization of flower resource. The aroma components in flower buds of three kinds of tea plants, Dayezhong, Zhongyezhong and Xiaoyezhong were analyzed by HS-SPME/GC-MS. The relative contents of the chemical constituents were determined by area normali-zation method. Totally 37, 32 and 45 aroma compounds were identified from Dayezhong, Zhongyezhong and Xiaoyezhon-gin Liubaowhich accounted for 99. 75 %, 99. 02% and 99. 45% of the total aroma components, respectively. The main constituents in the Dayezhong were Acetophenone, 4-Methyl-1,5-Heptadiene, Benzoic acid, methyl ester, Guaia-3,9-diene, cis-Linaloloxide, Cedarene,Methyl salicylate, d-Cadinene, 1-amino-Cyclopentanemethanol,In the Zhongyezhong were Acetophenone,Perillene,(1S)-(+)-3-Carene,(+)-α-Elemene,Benzoic acid ethyl ester,Seychellene,α-Copaene, Neoclovene,Ylangene,cis-Linaloloxideand acetophenone,Perillene,Ocimene,(+)-α-Elemene, 2-isopropyl-5-methyl-9-methylene-(+)-α-ElemenBicyclo[4.4.0]dec-1-ene, Cubenol,α-Acoradiene,α-Bisabolene,Ylangene,Benzoic acidethyl ester in the Xiaoyezhong. Acetophenone was the characteristic compounds among the three kinds of tea plants which con-stituted their unique aroma along with other components. The study provides scientific support for exploitation and utiliza-tion of flowers buds of the tea plants.%该研究采用顶空固相微萃取—气相色谱/质谱联用技术，对六堡本地三种茶树花的香气成分进行了分析。结果表明：大叶种茶树花中共鉴定出香气成分37种，主要为苯乙酮、4-甲基-1，5-庚二烯、苯甲酸甲酯、愈创木二烯、顺式芳樟醇氧化物、雪松烯、水杨酸甲酯、D-杜松烯、1-氨基-环戊醇、除虫菊酮，占总相对含量的89.48％；中叶种共鉴定出32种成分，主要为苯乙酮、紫苏烯、顺式-3-蒈烯、顺式α-榄香烯、苯甲酸乙酯、塞瑟尔烯、α-蒎烯、新丁香三环烯、衣兰烯、顺式芳樟醇氧化物，占总相对含量的83.88％；小叶种的茶树花中共鉴定出45种香气成分，主要为苯乙酮、紫苏烯、罗勒烯、顺式α-榄香烯、2-异丙基-5-甲基-9-亚甲基-二环[4.4.0]癸-1-烯、荜澄茄油烯醇、α-菖蒲二烯、α-红没药烯、衣兰烯、苯甲酸乙酯、白菖油萜和α-杜松烯，占总相对含量的82.34％。苯乙酮为三种茶树花共有的主要成分，分别占总相对含量的60.70％、42.46％和39.91％，这成分与其他成分一起构成了3个品种明显不同的茶树花花香。该研究结果为六堡茶树花的深加工提供了依据。