荧光密螺旋体抗体吸收实验在梅毒血清学实验中的应用

摘要

Objective Study the application of Fluorescent treponemal antibody absorption test(FTA-ABS) in the serological tests for syphilis. Methods 28 serum samples, which have been positive in the Treponema pallidum enzyme-linked immunosorbent assay(TP-ELISA), were tested again by Treponema pallidum particle assay(TPPA)、Fluorescent treponemal antibody absorption test(FTA-ABS) and Rapid plasma reagent test(RPR). Calculate and compare the detecting rates of the three methods. Results The positive rate of TPPA was 89.29%and of FTA-ABS was 82.14%, in which IgG antibody positive rate was 82.14%, and IgM antibody was 39.29%. RPR positive rate was 42.86%. The consistency was high between TPPA and FTA-ABS, however it was middle between RPR and FTA-ABS-IgM. Conclusions TP-ELISA can be used as the screening method to detect the antibody of syphilis. Other tests, like TPPA、FTA-ABS and RPR, and the clinical history are helpful in the dignosis of syphilis.%目的研究荧光密螺旋体抗体吸收实验（FTA-ABS）在梅毒血清学实验中的应用。方法选择梅毒螺旋体酶联免疫吸附试验阳性血清标本28份，分别用梅毒螺旋体明胶颗粒凝集试验、FTA-ABS、快速血浆反应素环状卡片试验复检。计算和比较三种方法的检测率。结果 TPPA阳性率为89.29%，FTA-ABS阳性率为82.14%，其中IgG型抗体阳性率为82.14%，IgM型抗体阳性率为39.29%，RPR阳性检出率为42.86%。TPPA法与FTA-ABS法有较高的一致性，而FTA-ABS法中IgM型抗体检出与RPR阳性检出相关性一般。结论 TP-ELISA适用于梅毒抗体的初步筛查，确诊需结合TPPA、FTA-ABS、RPR等实验方法及临床资料综合分析。