高同型半胱氨酸对小鼠足细胞CD2AP表达的影响

摘要

Objective To observation the effect of the alpha-lipoic acid on the expression of CD2AP mRNA in mouse podocytes cultured in homocysteine environment,To explore the protective effects of alpha-lipoic acid on podocyte. Methods The podocytes were cultured in high homocysteine and alpha-lipoic acid environment and collected respectively at 24、48、72h, the mRNA expression of CD2AP was examined by RT–PCR. Result The expression of CD2AP mRNA was decreased, and there is a certain timelines, alpha lipoic acid can resist the trend. Conclusion Alpha lipoic acid has protective effect on podocytes, and it can resist down-regulating the expression of CD2AP mRNA.%　　目的观察α-硫辛酸对高同型半胱氨酸（Hcy）作用下小鼠足细胞胞CD2AP mRNA表达的影响，探讨α-硫辛酸对足细胞的保护作用。方法将体外培养的足细胞分为正常组、Hcy组、Hcy+α-硫辛酸组，培养24、48、72h时分别收集细胞，RT-PCR法检测CD2AP mRNA表达。结果 Hcy组，足细胞CD2AP mRNA表达自24h开始下调（P＜0.05），且有一定的时效性，72h达到下调高峰（P＜0.01）；Hcy+α-硫辛酸组，48h时，CD2AP mRNA表达较Hcy组增高（P＜0.05），72h仍有上述差异（P＜0.05）。结论α-硫辛酸对足细胞有一定的保护作用，可能与其可对抗高同型半胱氨酸下CD2AP mRNA表达下调有关。