卡介苗各亚株间Rv1985c和Rv1986基因缺失的研究

摘要

Objective To clear BCG group on genetic differences in the inner Rv1985c and Rv1986 two of sites. Method Identified by PCR primers were designed based on Mycobacterium tuberculosis, BCG for 21 standard strains and clinical isolates Rv1985c and Rv1986 two of sites identified gene deletion. Result 21 BCG 10 missing Rv1985c gene, 11 missing Rv1986 gene. Conclusion Some strains of BCG in the two loci lack of alternative sites for future vaccine transformation, PCR method established in this study can be used in the clinical part of the BCG strain of Mycobacterium tuberculosisrapid identification.%　　目的明确卡介苗群体内在Rv1985c和Rv1986两个位点上的遗传差异。方法基于结核分枝杆菌设计引物，针对21株卡介苗标准株和临床株进行Rv1985c和Rv1986两个位点的PCR鉴定，鉴定基因的缺失情况。结果21株卡介苗中10株缺失Rv1985c基因，11株缺失Rv1986基因。结论卡介苗中部分菌株中两个基因位点的缺失，为今后的疫苗改造提供了备选位点，同时本研究中所建立的PCR方法也可以在临床上用于部分卡介苗菌株与结核分枝杆菌的快速鉴别。