RECOMBINANT PLASMID DNA PAD5-F CARRYING OF ADENOVIRUS TYPE 5 GENOME FRAGMENT WITH DELETION IN GENE E1B-55K AND STRAIN OF MUTANT ADENOVIRUS ADE 12 EXHIBITING SELECTIVE ANTITUMOR ACTIVITY

摘要

FIELD: biotechnology, medicine, oncology, genetic engineering, molecular biology. SUBSTANCE: invention presents recombinant plasmid DNA pAd5-f and mutant strain of human adenovirus Ade 12. Recombinant plasmid DNA carries fragment of genome of adenovirus type 5 with deletion in gene E1B- 55K. Mutant strain of adenovirus obtained by homologous recombination using plasmid pAs5-f exhibits the selective antitumor activity. Strain can be used as a base for the development of effective antitumor preparation. EFFECT: valuable medicinal properties of strain and plasmid. 2 cl, 3 dwg, 2 tbl, 4 ex