桑菊清解汤对慢性吸烟大鼠气道炎症的影响

摘要

目的 探究桑菊清解汤对慢性吸烟大鼠气道炎症的影响.方法 将慢性吸烟Wistar 大鼠随机分成：①吸烟对照组(吸烟组).②N-乙酰半胱氨酸组(NAC组).③低剂量桑菊清解汤组(低剂量组).④高剂量桑菊清解汤(高剂量组).⑤空白对照组(对照组).测定吸烟组、NAC组、低剂量组、高剂量组吸烟第60天的肺泡灌洗液(BALF)中白细胞计数和分类,同时测定BALF中白细胞介素-8(IL-8)含量.结果 慢性吸烟大鼠第60天BALF中白细胞总数、中性粒细胞数及IL-8水平均显著高于空白组；高、低剂量组与NAC组BALF中白细胞总数、中性粒细胞数和IL-8水平均显著降低.高、低剂量组与NAC组比较,P<0.05,有显著性差异,高、低剂量组比较,P<0.05,有显著性差异.结论 桑菊清解汤对慢性吸烟大鼠肺损伤的保护作用,可能与降低气道白细胞总数、中性粒细胞数和IL-8水平相关.且桑菊清解汤控制气道炎症的水平与剂量相关.%Objective To find out the effects of Sangju Qingjie Decoction on airway inflammation of chronic smoking rats. Method Randomly divide the Wistar rats into 5 groups, namely ①Smoking Control Group (Smoking Group), ②N-Acetyl Cysteine Group (NAC Group), ③Low Dose Sangju Qingjie Decoction Group (Low Dose Group), ④High Dose Sangju Qingjie Decoction Group (High Dose Group), ⑤Blank Control Group (Blank Group). After 60 days of smoking, the rats in Smoking Group, NAC Group, Low Dose Group and High Dose Group were given detection on the number and type of white cells in Broncho Alveolar Lavage Fluid (BALF) and the content of Interleukin-8. Result The numbers of white cells and neutrophile granulocyte, and the IL-8 level of all chronic smoking rats are significantly higher than those of Blank Group. The numbers of white cells and neutrophile granulocyte, and the IL-8 level of High Dose, Low Dose and NAC Group are significantly reduced. Comparing with the NAC Group, High Dose and Low Dose Group display a significant difference with P<0.05 which can also be found between the High Dose Group and Low Dose Group. Conclusion The result shows that the reason why Sangju Qingjie Decoction can protect the chronic smoking rats from chronic injury of lungs might rely on its effect of reducing the white cell and neutrophile granulocyte number and the IL-8 level. It also indicates that the effects of Sangju Qingjie Decoction on airway inflammation relate to the dose.