乙型肝炎病毒大蛋白检测的临床意义

摘要

目的：探寻乙型肝炎患者血清中乙型肝炎病毒表面大蛋白（LHBs）表达量的变化及其与病毒载量的相关性。方法对临床确诊为慢性乙型肝炎的170例病例及28例健康对照血清样本分析，ELISA检测乙型肝炎表面大蛋白的含量、实时定量PCR检测乙型肝炎病毒。结果乙型肝炎病毒表面大蛋白在乙型肝炎患者的血清中表达，在非乙型肝炎的患者中不表达，并且和乙型肝炎病毒的载量成正相关。结论乙型肝炎病毒表面大蛋白的表达，可以作为临床感染乙型肝炎病毒及临床疗效判断的参考依据。%Objective To find the relationtion of the large protein on the surface of hepatitis B virus(LHBs) and loading of hepatitis B virus in the serum . Methods Testing the concentration of LHBs by ELISA and loading of hepatitis B virus by real-time PCR in serum of 170 patients and 28 controls.Result There are some LHBs in the serum of patients with hepatitis B ,as the same time they are positive correlation and there are not any LHBs in the healthy control. Conclusion The large protein on the surface of hepatitis B virus could regard as a marker thought which indicate the infection hepatitis B virus and effect of the treatment.