红豆杉中紫杉醇的提取和纯化工艺研究

摘要

基于紫杉醇的抗癌功效，探究何种条件（乙醇浓度，固液比，提取时间，提取次数，提取功率，温度，颗粒直径，溶媒，提取方法，提取部位）下，能在有限的红豆杉中最大量的提取紫杉醇很有必要。在单因素条件的基础上，选取预处理中影响紫杉醇提取的4个因素（固液比，提取时间，超声波功率，温度）进行4因素3水平（L9（34））正交实验。然后对K值进行分析，得出结论：最优的超声波预处理实验组合为温度50℃、固液比为1：35、超声波提取时间为40 min、超声波功率为70％。最后采取氧化铝柱层析进一步除杂，通过高效液相色谱结果，对提取纯化的效果进行分析。%based on Paclitaxel anti-cancer efficacy, it is necessary to find out that, under which conditions a large number of Paclitaxel can be extracted from Taxus chinensis, such as ethanol concentration, solid-liquid ratio, extraction time, extraction times, extraction power, temperature, particle diameter, solvent, extraction method, extraction parts, etc.Based on single factor conditions, 4 factors, solid-liquid ratio, extraction time, ultrasonic power, temperature, which influenced the selection of taxol extraction pretreatment, were used in L9(34 ) orthogonal experiment.Analyzing the K value, it was concluded that the best optimal ultrasonic pretreatment experiment combination was as follows: the temperature was 50℃, the ratio of solid to liquid was 1:35, ultrasonic extraction time was 40 min, ultrasonic power was 70%. Using alumina column chromatography to impurities further removement, under the high performance liquid chromatography results, the effect of the extraction and purification was analyzed.