Purification of Taxol from Taxus Plants by Chromatography

摘要

The present invention relates to a method for purifying Taxol from Taxus genus plants. More specifically, the present invention provides a high-purity taxol with high recovery rate by a simple process of separating the crude taxol by solvent extraction and fractional precipitation of pretreatment of the taxus plant, and then purifying the crude taxol by fractional precipitation and crystallization. It relates to a method for purifying economically. The method for purifying Taxol from Taxus plants according to the present invention is a method of purifying plant cells obtained by grinding and pulverizing dried dried leaves or bark of attention as starting materials, or by culturing the tissue of interest in a fermenter and recovering plant cells. Using a sample, mixing a sodium chloride aqueous solution with a sample of a plant of the genus Taxus, filtering, stirring by adding methanol, vacuum filtration and concentrating the filtrate under reduced pressure; Methylene chloride was added to the concentrate, followed by extraction with purified water. The mixture was extracted, and then the water layer was removed. Then, the solvent was added to the concentrated solution, which was then concentrated under reduced pressure. The mixture was extracted, and the organic layer was removed. Removing silver, concentrating under reduced pressure, and then adding the concentrated solution to hexane to collect and precipitate the precipitate precipitated and dried to extract crude taxol; Adding methanol to the crude taxol to dissolve it, adding purified water, leaving it for 1 to 2 days, filtering and drying the filtrate under vacuum to obtain taxol powder; And dissolving the taxol powder in a mixed solvent of ethyl acetate, methanol and water, and leaving it at 0 to 8 ° C. for 1 to 2 days to crystallize the taxol and to filter the filtrate by vacuum drying at 20 to 30 ° C. for 1 to 3 hours. do.