Objective To establish type 1 diahetic BALB/c mouse models and investigate the significance of CD4+ CD25 + regulatory T cells ( Treg ) in TIDM. Methods Sixty BALB/c mice were randomly divided into four groups ( n =15 ). BALB/c mouse diabetic models were established with single intraperitoenal ( ip ) injection of 150 mg/kg of streptozotocin ( STZ ) and 5 separated ip injection of 40 mg/kg of STZ in Group A and Group C, respectively, while controls were set up with corresponding injections of citrate buffer solution in Group B and Group D, respectively. Blood sugar level was measured. The frequency of CD4+ CD25 + Treg cells in peripheral blood was determined hy flow cytometry. Results Type Ⅰ diabetes mellitus ( TIDM ) was observed in 86. 67％ and 93. 33％ mice of Group A and Group C, respectively , with suspended high blood glucose since the 2lth day. No significant difference was revealed between Group A and C ( P ＞ 0. 05 ). The peripheral CD4 + CD25 + Treg cell proportion was significantly lower in model groups than that in control group[ ( 4. 05 ±0. 37 )％ vs ( 8. 68 ±0. 45 )％ , P ＜0. 05 ]. Conclusion Single intraperitoneal injection of STZ is an effective method in construction of BALB/c TIDM mouse.%目的 探讨链脲佐菌素(streptozotocin,STZ)诱导BALB/c小鼠1型糖尿病模型的方法,检测糖尿病模型中CD4+CD25+调节性T细胞(regulatory T cell,Treg)的变化及意义.方法 将60只雄性BALB/c小鼠随机分为A、B、C、D 4组,每组15只,A组采用一次性腹腔注射150 mg/kg STZ,B组采用一次性腹腔注射与A组等量无菌柠檬酸缓冲液,C组采用200 mg/kg STZ分5次腹腔注射,D组采用与C组等量无菌柠檬酸缓冲液分5次腹腔注射,监测不同时点空腹血糖变化,A、B、C、D 4组均自由饮食.用流式细胞术检测成模前后外周血Treg细胞数的变化.结果 A组糖尿病成模率为86.67%,血糖在3周后稳定在较高水平且无明显下降;C组糖尿病成模率为93.33%,血糖的动态变化与A组相似;两组糖尿病成模率相比差异无统计学意义(P＞0.05);B、D组无糖尿病成模;非成模组与成模组外周血CD4+CD25+Treg分别为(4.05±0.37)%和(8.68±0.45)%,差异有统计学意义(P＜0.05).结论 采用一次性腹腔注射STZ是构建BALB/c小鼠1型糖尿病模型较为理想的方法.外周血CD4+CD25+Treg的比例可作为1型糖尿病模型构建成功的参考指标.
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