N-乙酰半胱氨酸对辐照引起的大鼠肠隐窝上皮细胞损伤的减轻作用

摘要

目的 研究N-乙酰半胱氨酸(NAC)对辐照引起的肠隐窝上皮细胞IEC-6的损伤的防护作用及可能机制.方法 将IEC-6细胞分为3组:正常组(无辐照),辐照组(6 Gy辐照)和NAC治疗组(6 Gy辐照+NAC 10 mg/mL).IEC-6细胞辐射损伤模型采用总剂量为6 Gy的钴-60照射,用DCFH-DA测定细胞内的活性氧(ROS)和丙二醛(MDA)的含量以及超氧化物歧化酶(SOD)的活力,用Annexin V-FITC和碘化丙啶进行细胞凋亡的检测,同时用Hoechst 33342进行细胞凋亡的检测,同时进一步检测IEC-6细胞的增殖能力.结果 NAC减少了辐照引起的ROS和MDA的过量产生以及SOD的过度消耗,同时流式细胞仪和核染色的结果显示,NAC抑制了辐照引起的细胞凋亡和增殖活力的下降.结论 NAC可以减轻辐照引起的IEC-6细胞的损伤.%Objective To investigate the protective effect of N - acetylcysteine on γ - radiation - induced intestinal epithelial cell ( IEC - 6 ) damage in rats. Methods IEC - 6 cells were divided into three groups: normal group ( Group N ), radiation group ( 6 Gy radiation, Group R ) and NAC treatment group ( 6 Gy radiation + NAC ( 10 mg/mL ), Group E ). Reactive oxygen species ( ROS ) in cultured IEC -6 cells was measured using Fluorescent Probe 2', 7'-di-chlorofluorescin diacetate ( DCFH-DA ). Malonaldehyde ( MDA ) and superoxide dismutase ( SOD ) assays were also performed at 24hrs post-radiation. Cell apoptosis was determined by annexin V-APC and propidium iodide ( PI ) stain u-sing the Apoptosis Detection Kit. Furthermore, the cells were stained with Hoechst 33342 ( apoptotic cell ) to visualize the nuclei. Alamarblue cell viability reagent was used to determine the cell viability exposure to γ-radiation. Results NAC reduced the y - radiation - induced over - production of ROS and MDA, and maintained the SOD activity in IEC - 6 cells. In addition, the radiation - induced apoptosis and cell viability reduction was alleviated by NAC treatment. Conclusion NAC could significantly reduce y - radiation - induced IEC - 6 damage.