过氧化物酶体增殖物激活受体γ在反流性食管炎、Barrett食管和食管腺癌中的表达

摘要

Objective To investigate the PPARγ expression and cell proliferation in reflux esophagitis ( RE ), Barrett esophagus ( BE ) and esophageal adenocarcinoma ( EA ). Methods The expression of PPARγ and PCNA were determined by immunohistochemistry technique and Western blot in patients with RE, BE and EA, and the normal controls , of which the esophageal mucous membrane biopsy were obtained by gastroscopy. Results The PPARγ positive rates in RE, BE and EA were 25% , 30% and 33. 3% , respectively; significantly higher than that in normal controls ( 10% , P <0. 05 ). Moreover, the PPARγ protein absorbance ratios in RE, BE and EA were 0. 28 ±0. 15, 0. 21 ±0. 18 and 0. 32 ± 0. 13 , respectively; significantly higher than that in normal controls ( 0. 08 ± 0. 06, P < 0. 05 ). However, there was no significant difference among RE, BE and EA groups in PPARγ expression. The PCNA positive rates in RE, BE, EA and control groups were 25% , 60% , 75% and 100% , respectively. Conclusion There are the progressing cell proliferation and up - regulation of PPARγ protein while the malignancy progression of esophagus, suggesting PPARγ is involved in the cell proliferation regulation in the pathogenesis of reflux esophagitis, Barrett esophagus and esophageal adenocarcinoma.%目的 观察反流性食管炎、Barrett食管和食管腺癌中食管上皮细胞过氧化物酶体增殖物激活受体γ(PPARγ)的表达及细胞增殖的变化特点.方法 胃镜下取20例反流性食管炎(RE组)、20例Barrett食管(BE组)和6例食管腺癌(EA组)和20例慢性浅表性胃炎(正常对照组)的食管黏膜组织进行活检,免疫组化法和免疫印迹法测定各组黏膜组织食管上皮细胞PPARγ和增殖细胞核抗原(PCNA)的蛋白表达.结果 免疫组化法显示RE、BE、EA组PPARγ的阳性表达率分别为25%、30%、33%,均高于正常对照组的10%(P<0.05);免疫印迹法显示RE、BE、EA组的PPARγ蛋白吸光度比值分别为0.28±0.15、0.21±0.18、0.32±0.13,均高于正常对照组的0.08±0.06(P<0.05);RE、BE、EA组3组间的PPARγ表达差异无统计学意义(P>0.05).免疫组化法显示正常对照组、RE组、BE组、EA组PCNA的阳性表达率分别为25%、60%、75%、100%.结论 从正常食管到反流性食管炎、Barrett食管、食管腺癌,食管上皮的细胞增殖明显增加,伴随着PPARγ蛋白的表达增加,提示PPARγ可能参与了反流性食管炎、Barrett食管、食管腺癌发病过程中细胞增殖的调控.