转酪氨酸激酶C基因神经干细胞移植对脊髓损伤后肿瘤坏死因子-α的影响

摘要

Objective To investigate the therapeutic effect of trans - TrkC gene neural stem cells(NSCs)on the recovery of neural function after spinal cord injury. Methods A total of 30 SD rats were randomly divided into 3 groups:Control group(Group A),NSCs transplantation group(Group B),trans - TrkC gene NSCs transplantation group(Group C). Cell transplantation was performed 1 day after set - up of animal model. Spinal cord injury was examines via TNF -α protein levels,pathological observation and apoptosis analysis. Results Expression of tumor necrosis factor - α in Group B and A was significantly higher than Group C(F = 121. 145,P < 0. 01). There was no significant difference be-tween Group A and B in TNF - α expression within 24 h(P > 0. 05). According to HE and NISSLE,the severest injury of neural cells was observed in Group A,followed by Group B and C. The most significant cell apoptosis was revealed in Group C,followed by Group B and A. Conclusion Trans - TrkC gene NSCs transplantation in injured spinal cord of rats can down - regulate TNF - α,suggesting reduction in secondary spinal cord injury and neuron regeneration.%目的：探讨转酪氨酸激酶 C（TrkC）基因神经干细胞（NSCs）移植对脊髓损伤的作用。方法30只Wistar 大鼠，随机分为3组（每组10只），常温下对照组只进行单纯阻断降主动脉群体；单纯移植组阻断降主动脉，同时，进行单纯 NSCs 移植；TrkC 组阻断降主动脉，同时，进行转 TrkC 基因 NSCs 移植。于手术后1 d 进行细胞移植。通过检测肿瘤坏死因子-α（TNF -α）及病理学分析、细胞凋亡检测等方法评估脊髓损伤情况，及评估转 TrkC基因 NSCs 移植对脊髓损伤后 TNF -α的影响。结果对照组和单纯移植组损伤脊髓 TNF -α浓度较 TrkC 组明显增加（F =121.145，P <0.01），单纯移植组在移植后24 h TNF -α浓度与对照组比较并无减少（P >0.05），24 h后才逐渐受到抑制，较对照组减少（P <0.01）；而 TrkC 组 TNF -α浓度较单纯移植组减少（P <0.01），HE、Nissle染色显示神经细胞损伤情况：对照组>单纯移植组> TrkC 组，TrkC 组损伤程度最轻。细胞凋亡情况：TrkC 组<单纯移植组<对照组，单纯移植组、TrkC 组细胞凋亡减少。结论转 TrkC 基因 NSCs 移植到脊髓受损的大鼠身上，可使受损脊髓局部的 TNF -α表达程度降低。这可能是 TrkC 基因 NSCs 移植改变脊髓损伤的微环境，使干细胞更好地分化，有效减少脊髓继发损伤，修复受损神经，促进大鼠脊髓修复的机制。