Objective To study the expression of LunX in human non-small cell lung cancer ( NSCLC) tissue and its correlation to the micrometastasis.Methods A total of 54 patients with NSCLC were enrolled and divided into two groups:the metastasis group ( metastasis in lymph nodes in the hilus of lung and the mediastinum) and the non-metasta-sis group.Twenty-one patients with pulmonary benign diseases were also enrolled as control group.Real-time fluores-cence quantitative polymerase chain reaction ( FQ-PCR) , Western blotting and immunohistochemistry methods were used to analyze the expression of LunX.Results According to FQ-PCR, the expression of LunX gene in the cancer tissues, adjacent tissues and lymph node of the metastasis group was significantly higher than non-metastasis group (1.65 ±0.18 vs.0.71 ±0.08, 1.14 ±0.23 vs.0.57 ±0.06, and 2.57 ±0.38 vs.1.89 ±0.53, respectively, P<0.01).According to Western blotting, the expression of LunX channel in cancer tissues and lymph node of the metastasis group were signifi-cantly higher than non-metastasis group (1.67 ±0.27 vs.0.84 ±0.27, and 3.12 ±0.31 vs, 1.97 ±0.48, respectively, P<0.01) .So was according to immunohistochemistry (4.04 ±0.41 ×106 vs.3.45 ±0.43 ×106 , and 3.95 ±0.69 ×106 vs.2.47 ±0.73 ×106, respectively, P<0.01).There was no LunX expression in non-cancer lung tissues.Conclusion LunX is expressed in the cancer tissues, adjacent tissues and lymph node in NSCLC patients, suggesting an important role in development of NSCLC.%目的：研究肺组织特异性X蛋白（ LunX）在非小细胞肺癌（ NSCLC）患者中的表达，探讨LunX与NSCLC患者肿瘤微转移的关系。方法收集54例NSCLC患者，转移组（ n＝34）为NSCLC并肺门纵隔淋巴结转移，非转移组（n＝20）为NSCLC无肺门纵隔淋巴结转移患者，另选取同期胸部良性病变行手术患者21例为对照组。采用荧光定量聚合酶链式反应法（ FQ－PCR）、蛋白印记法和免疫组织化法检测54例NSCLC患者癌组织、癌旁组织及淋巴结中LunX基因及蛋白的表达。结果 FQ－PCR结果显示转移组患者癌组织、癌旁组织及癌转移淋巴结中LunX mRNA表达量均明显高于非转移组（ P＜0.01）；蛋白印记结果显示转移组癌组织及癌转移淋巴结中LunX蛋白相对表达量较非转移组明显上调（ P＜0.01）；免疫组织化学结果显示转移组癌组织及癌转移淋巴结LunX蛋白表达量明显高于非转移组（P＜0.01），两组癌旁组织在蛋白印迹及免疫组化表达上差异无统计学意义，而对照组肺组织及肺门纵隔淋巴结无LunX mRNA及蛋白表达。结论 LunX在NSCLC患者癌组织、癌旁组织及淋巴结中均有表达，其表达的上调可能参与NSCLC患者肿瘤微转移的发生发展过程。
展开▼
