Objective To investigate the expression of PKCzeta in prostate cancer and its correlation with the pro liferation of prostate cancer cell lines.Methods The expression of PKCzeta was measured by immunohistochemistry in 19 cases of prostate cancer and 4 cases normal prostate tissues.According to the tumor grade,the prostate tissues were divided into 3 groups.Hilymax was used to transfect the plasmid of PKCzeta in PC3 and DU145 cell lines.The mRNA and protein levels were assessed by RT-QPCR and Western Blot.The ability of cell proliferation was evaluated in PC3 and DU145 cells by CCK-8 kit and plate colony forming.Results The expression of PKCzeta was significantly increased in prostate cancer (P ＜0.01) and had higher level in low tumor grade (Grade 1) (P ＜0.001).The mRNA and protein levels of PKCzeta showed a higher level in DU145 cells,which contributed to a decrease in proliferation.The proliferation ability of PCa cells was inhibited by PKCzeta overexpression.Conclusion A negative correlation exists between PKCzeta expression and tumor grade,which suggested PKCzeta may play a role to protect from further progression of prostate cancer and control the proliferation of prostate cancer cell lines.%目的 检测PKCzeta在不同分级前列腺癌(PCa)组织的表达情况,并探究PKCzeta的表达对前列腺癌细胞系增殖的影响.方法 采用免疫组织化学染色法检测19例前列腺癌组织和4例正常前列腺组织的PKC-zeta表达情况,并按肿瘤分级分为3组,进行PKCzeta表达的差异性分析;应用Hilymax试剂转染PKCzeta质粒(CA-PKCzeta)过表达前列腺癌细胞PKCzeta;通过实时荧光定量PCR(RT-qPCR)和Western blot分别检测PC3和DU145细胞内PKCzeta的mRNA和蛋白水平;利用平板克隆形成试验和CCK-8试剂盒检测PC3和DU145细胞增殖能力的差异以及转染PKCzeta质粒后细胞增殖的变化.结果 PKCzeta在前列腺癌组织中的表达高于正常前列腺组织(P＜0.05),而且低分级(1级)前列腺癌PKCzeta的表达高于高分级(2～3级)前列腺癌(P＜0.05).DU145细胞的PKCzeta表达高于PC3细胞,而其增殖速度较PC3细胞低;过表达PKCzeta使前列腺癌细胞系的增殖受抑.结论PKCzeta与肿瘤分级存在负相关关系,在癌症早期可能有一定的保护作用,且过表达PKCzeta能抑制前列腺癌细胞的增殖.
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