miR-21在前列腺癌中的表达及抑制其表达对前列腺癌细胞增殖凋亡的影响

摘要

目的 探讨抑制前列腺癌细胞中miR-21基因表达对癌细胞增殖和凋亡的影响及机制.方法 RT-PCR检测前列腺癌及癌旁组织中miR-21基因的mRNA表达;空白对照组(Control)、阴性对照组(NC)和miR-21 in-hibitor组转染人前列腺癌PC-3细胞,48 h后RT-PCR检测各组细胞中miR-21基因的mRNA表达;CCK8实验检测细胞增殖;流式细胞仪检测细胞凋亡;Western blot检测ki67、Cleaved caspase3、β-catenin、Cyclin D1蛋白表达.结果 前列腺癌组织中miR -21 的mRNA 表达显著高于癌旁组织(P 0. 05),miR -21 inhibitor 组细胞存活率及ki67?β - catenin?Cyclin D1 蛋白表达显著低于对照组,细胞凋亡率及Cleaved caspase3 蛋白表达显著高于对照组(P 0.05). The cell survival rate and Ki67,β-catenin,Cyclin D1 pro-tein expression was significantly lower than the control group. The apoptosis rate and the expression of Cleaved Caspase3 protein was significantly higher than the control group(P <0.01). Conclusion The inhibition of miR-21 gene expression in prostate cancer can reduce the prolifera-tion of cancer cells and induce apoptosis,which may be related to the inhibition of Wnt/β-catenin signaling pathway.