干旱胁迫下多年生黑麦草对污泥处理的生理生化响应

摘要

为了探究污泥对干旱胁迫下多年生黑麦草(Lolium perenne)的生长及生理影响,采用裂区试验设计,主处理包括正常浇水和干旱处理2个水平;副处理为不同的氮源即全污泥处理(利用污泥提供黑麦草所需75 mg/kg的氮量)、半污泥处理(利用污泥提供黑麦草所需37.5 mg/kg的氮量,剩余37.5mg/kg氮量由硝酸铵提供)和对照(利用硝酸铵提供黑麦草所需75 mg/kg的氮量).结果表明:正常浇水和灌溉下,全污泥和半污泥处理能够显著提高黑麦草的坪观质量、叶片叶绿素含量和净光合速率(P＜0.05);在干旱处理后期,全污泥和半污泥处理可以显著提高黑麦草叶片细胞膜稳定性(P＜0.05),全污泥处理可显著提高黑麦草SOD、CAT、APX、POD活性(P＜0.05).表明施用污泥可以提高黑麦草抗氧化酶活性,促进黑麦草在干旱条件下的生长.%In order to investigate the physiological effects of biosolid on Lolium perenne under drought stress,a split-plot design was used with the main plots of solid moisture and the subplots of biosolids.Main plot treatments were conducted included two levels of normal watering and deficit irrigation.Subplot treatments were different nitrogen source:biosolid 1 × rate (75 mg/kg of nitrogen required for the use of biosolid),biosolid 0.5×rate (37.5 mg/kg of nitrogen required for the biosolid,and the remaining 37.5 mg/kg of NH4NO3 solution) and control (75 mg/kg of nitrogen required for the use of NH4 NO3 solution).The results showed that under both normal watering and deficit irrigation,turf quality,leaf chlorophyll content and net photosynthetic rate were significantly improved by biosolid 1 × rate and biosolid 0.5× rate (P＜0.05).The activity of SOD,CAT,APX and POD in ryegrass significantly increased by biosolid 1 × rate (P＜0.05),and the effect of biosolid 1 × rate and biosolid 0.5 × rate could significantly improve the cell membrane stability of Lolium perenne (P＜ 0.05).These results indicated that the application of biosolid can improve the growth of ryegrass in drought conditions by increasing the antioxidant enzyme activity.