Objective To discuss the mechanism of the treatment in glioma with compound Chinese medicine optimization of Kangliuwan ( KLW-4). Methods BALB/c nude mice transplantation tumor animal models of U87 glioma cells was established, and randomly divided them into 5 groups: model control group, temozolomide (TMZ) group (positive drug control group), KLW-4 high, medium and low dose groups, with 12 animals in each group. After 20 days of continuous drug administration, by observing the general conditions of the mice and the pharmacodynamic indicators such as the medical effects on the volume and weight of glioman, using Western blot method to detect the expression of apoptosis related Bax, Bcl-2 protein, the mechanism of action of KLW-4 in the treatment of glioma was explored. Results Compared with model control group, four days after the administration, tumor volume of all dose groups de-creased significantly. Eight days after the treatment, tumor volume of middle dose of KLW-4 group and positive control drug group decreased significantly. The difference was statistically significant (P<0. 05). For 12 days, tumor volume of all dose groups significantly decreased. After 16 days, tumor volume of high, middle dose of KLW-4 groups and positive control drug group decreased significantly. 20 days later, tumor volume of high, middle dose of KLW-4 groups and positive control drug group significantly decreased. Compared with model control group, the expression of Bcl-2 was down-regulated significantly in all drug-ad-ministered groups, and the expression of Bax was up-regulated significantly in positive control group. The difference was statistically significant (P<0. 05). Conclusion KLW-4 has good therapeutic effects on U87 BALB/c nude mice transplantation tumor animal models and can reduce tumor volume. Besides, KLW-4 may be related to the promotion of apoptosis.%目的 探讨中药复方抗瘤丸优化方(KLW-4)治疗神经胶质瘤的作用机制.方法 建立BALB/c裸小鼠U87移植瘤动物模型,并将造模后的动物随机分为五组:模型对照组、替莫唑胺组(阳性药对照组)、抗瘤丸优化方高剂量组、中剂量组、低剂量,每组12只.连续给药20天后,通过观察给药后裸鼠一般情况,以及药物对裸鼠胶质瘤体积变化的影响等药效学指标,并运用Western Blot法检测与凋亡相关的Bax、Bcl-2蛋白的表达,进而评价抗瘤丸优化方治疗神经胶质瘤的作用机制.结果 与模型对照组比较,给药4天后,所有给药组肿瘤体积均显著降低;给药8天后,中剂量组和阳性药对照组肿瘤体积显著降低,差异有统计学意义(P<0. 05);给药12天后,所有给药组肿瘤体积显著减小;给药16天后,高、中剂量组,阳性药对照组肿瘤体积显著降低;给药20天后,高、中剂量组,阳性药对照组肿瘤体积显著降低.与模型对照组比较,所有给药组均都能明显下调Bcl-2的表达,阳性药对照组能显著上调Bax的表达,各给药组Bcl-2/Bax的比例减小,差异有统计学意义(P<0. 05).结论 抗瘤丸优化方对U87裸鼠移植瘤动物模型有良好的治疗作用,可减少肿瘤体积.同时,抗瘤丸优化方治疗胶质瘤的可能与促进细胞凋亡有关.
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