复方芪术汤对四氯化碳致大鼠肝纤维化的影响研究

摘要

目的探讨复方芪术汤对四氯化碳(CCl4)致大鼠肝纤维化药效的干预作用.方法Wistar雄性大鼠30只,随机分为正常组10只和四氯化 碳处理组(20只),四氯化碳1 ml/kg,每周2次腹腔注射,正常组注射等量的生理盐水.造模的第8周,四氯化碳处理组大鼠随机分为复方芪术 汤组和模型组,每组10只.在继续造模的同时,复方芪术汤组给予70千克成人的10倍量灌胃,模型组给予等量的生理盐水灌胃,给药4周.12周 末处死全部大鼠,留取肝组织样本和血清,测定谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)、总胆红素(TBiL).结果四氯化碳 造模12周,模型组血清ALT、AST活性显著升高;ALB含量显著下降.血清TBiL含量显著升高.与模型组相比,复方芪术汤组大鼠血清ALB含量显 著升高,血清ALT和AST活性显著降低(P<0.01).但复方芪术汤对血清TBiL降低不明显.病理组织学显示,模型组假小叶形成,肝脏纤维化程 度分级均为2、3 级;复方芪术汤干预组肝小叶结构不同程度被破坏,肝脏纤维化程度分级大部分在1、2 级.结论复方芪术汤可以显著改善大 鼠肝功能,对CCl4引起的慢性肝纤维化大鼠有很好的降低肝纤维化程度的作用.%Objective To investigate the therapeutic effects of Compound Qizhu dedoction on carbon tetrachloride ( CCl4 ) -induced liver fibrosis in rats. Methods 30 Wistar male rats were randomly divided into normal group (10) and CCl4-treated group ( 20) , CCl4 ( 1 ml/kg) was administrated intraperitoneal to CCl4 -treated rats for twice a week for 12 weeks. The normal group injected the same amount of saline. At the first day of the 8 th, the CCl4 rats were randomly divided into Compound Qizhu de-doction group (10) and model group ( n= 10). Both groups continued to receive weekly CCl4 treatment for another 4 weeks in addition to daily administration of either saline or Compound Qizhu dedoction, the model group given the same amount of saline. All rats were sacrificed at the 12 weeks. Results Compared with normal rats, alanine aminotransferase ( ALT) and aspartate aminotransferase ( AST) activity and total bilirubin (TBiL) in the serum was significantly increased in the 12 weeks model rats (P<0. 01) , the albumin (ALB) content decreased remarkably (P<0. 01). Compared with model group, ALB content sig-nificantly increased in the Compound Qizhu dedoction rats, and ALT and AST activity was significantly decreased (P <0. 01) , Compound Qizhu dedoction could decrease Tbil content in the serum but there were not significant (P>0. 05). Compared to 12 weeks CCl4 rats, hepatic pathological changes and liver functions were improved significantly in Compound Qizhu dedoction. Conclusions Compound Qizhu decoction exerts significant inhibition on CCl4-induced cirrhosis formation in rats.