利湿活血方对高尿酸血症大鼠尿酸及肾脏抗氧化能力的影响

摘要

Objective To study the effects and mechanism of different doses of Lishi Huoxue prescription on uric acid, renal antioxidant capacity in hyperuricemia rats induced by yeast extract and adenine.Methods Male SD rats were randomly divided into 7 groups: the blank group, model group, Simiaowan group, Benzbromarone group Lishi Huoxue high, medium, and low-dose(7.74 g/kg、3.87 g/kg、1.935 g/kg) group.In addition to the blank group, the rest groups were intragastric administration with yeast extract and adenine for two weeks.After the model was established successfully, the appropriate drugs were administered to the rats of the administration groups, the blank group and model group were given distilled water for 14 days.uric acid(UA), xanthine(XOD), adenosine deaminase(ADA), superoxide dismutase(SOD), total antioxidant capacity(T-AOC), malondialdehyde(MDA) were detected.Results Compared with the model group, the UA, XOD and ADA of Lishi Huoxue high and medium groups were obviously reduced(P<0.01, P<0.05);SOD, T-AOC activity in Lishi Huoxue high, medium and low-dose groups were increased in serum and renal while MDA content was decreased, results had significant difference (P<0.01, P<0.05).Conclusion The Lishi Huoxue prescription can reduce the production of serum uric acid, and can improve the renal antioxidant capacity in hyperuricemia rats.The Lishi Huoxue can achieve the good therapeutic effect in the treatment of hyperuricemia.%目的 探讨不同剂量的利湿活血方对酵母膏合腺嘌呤诱导的高尿酸血症大鼠尿酸生成、肾脏抗氧化能力的影响.方法 将70只雄性SD大鼠随机分成空白组、模型组、四妙丸组、苯溴马隆组及利湿活血方高、中、低(7.74 g/kg、3.87 g/kg、1.935 g/kg)剂量组,共7组,每组10只.除空白组外,其余各组利用酵母膏联合腺嘌呤连续灌胃14天,制备高尿酸血症大鼠模型.造模成功后,各药物组给予相应药物,空白组和模型组给予等体积蒸馏水,连续灌胃14天后取材,检测各组大鼠血清中尿酸(uric acid,UA)、黄嘌呤氧化酶(xanthine oxidase, XOD)、腺苷脱氨酶(adenosine deaminase, ADA),血清及肾脏组织中过氧化物歧化酶(superoxide dismutase, SOD)、总抗氧化能力(total antioxidant capacity, T-AOC)、丙二醛(malondialdehyde, MDA)水平. 结果 与模型组比较,利湿活血方高剂量组、利湿活血方中剂量组可减少血清中UA含量,降低XOD、ADA活性,差异有统计学意义(P<0.001、P<0.01、P<0.05);利湿活血方高、中、低剂量组均可升高血清及肾脏组织中SOD、T-AOC活性,降低MDA含量,差异有统计学意义(P<0.001、P<0.01、P<0.05). 结论 中药利湿活血方有显著的减少高尿酸血症大鼠血尿酸的生成,增强高尿酸血症大鼠肾脏抗氧化和清除氧自由基能力的作用,对高尿酸血症的综合治疗有较好的效果.