Objective To study the diuretic effects of the Zhuang-medicine Inula cappa Alcohol Extract and the effects on hyperuricemia(HUA) induced by oteracil potassium in mice. Methods The urine volume of the mice was measured by the metabolic cage method, and the diuretic effect of the Zhuang-medicine Inula cappa Alcohol Extract was observed. HUA model was induced by oteracil potassium. HUA mice were treated with 1.0,2.0,4.0 g/kg Inula cappa Alcohol Extract for 7 days. Uricase Inhibitor method was used to observe the change of serum uric acid and liver Xanthine oxidase (XOD) in HUA mice.Results The amount of urine volume in low, medium and high dose groups were significantly higher than the blank control group in 1~2h or 2~3h(P<0.05). After treatment with Inula cappa alcohol extract,level of serum uric acid was markedly reduced by approximate 29.18%、40.88%、45.36%( P<0.01) compared with that of model group. Meanwhile,XOD activity in serum were markedly decreased by approximate 26.66%、34.24%、39.47%(P<0.05), as well as the liver XOD activity were markedly decreased 16.99%、22.70%、31.69% (P<0.05,P<0.01). Conclusion Inula cappa alcohol extract has a certain diuretic effect on mice,and can significantly reduce the level of blood uric acid in hy-peruricemia mice,and its mechanism is closely related to the inhibition of xanthine oxidase activity.%目的 观察壮药大力王醇提物的利尿作用,及对氧嗪酸钾诱导的小鼠高尿酸血症的影响.方法 采用小鼠代谢笼法测量给药后各组小鼠的排尿量,观察大力王醇提物的利尿作用,制备氧嗪酸钾诱导的小鼠高尿酸血症模型,连续7天灌胃给予大力王醇提物1.0、2.0、4.0 g/kg,采用尿酸酶抑制剂法观察各组小鼠血清尿酸及肝脏XOD活性.结果 小鼠给药后1~2小时和2~3小时,低、中、高剂量组的排尿量显著高于空白对照组,差异均有统计学意义(P<0.05);给予大力王醇提物灌胃后与模型组相比高尿酸血症模型小鼠血清中尿酸含量显著减少29.18%、40.88%、45.36%(P<0.01),血清中黄嘌呤氧化酶的活性显著降低26.66%、34.24%、39.47% (P<0.01),同时肝脏中黄嘌呤氧化酶的活性也显著降低16.99%、22.70%、31.69% (P<0.05).结论 大力王醇提物对小鼠有一定的利尿作用,大力王醇提物可显著降低高尿酸血症模型小鼠的血尿酸水平,且其作用机制与抑制黄嘌呤氧化酶的活性密切相关.
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