蝴蝶兰丛生芽增殖培养条件优化研究

摘要

Adventitious shoots induced from the peduncle auxiliary buds of Phalaenopsis ‘Lauluns’ were used for the study.The physical segmentation,placement mode and concentration of inorganic salt and organic additives all showed significant effects on the proliferation of Phalaenopsis with clustered shoots.Among the treatments,the greatest average number of inductive buds occurred when a bud was excised at the growth point and then grafted horizontally (PSP-3).However,the buds death rate was as high as 23.3％.In contrast,when the double buds were used,and followed by the horizontal grafting (PSP-6),the budding was high,but the death rate was low.The inorganic salt concentration between 1/3 MS to 1/2 MS was found to be suitable for the propagating with strong seedling growth.Addition of coconut juice at 15％ (v/v) level was desirable for the Phalaenopsis cluster buds proliferation,while 150 g · L-1 potato juice or banana puree could be applied as low cost organic additives in the culture medium.%以蝴蝶兰品种‘Lauluns’花梗腋芽诱导出的不定芽为试验材料,研究芽体物理切分与摆放方式、不同无机盐浓度以及有机添加物对蝴蝶兰丛生芽增殖的影响.研究结果表明:不同的芽体物理切分与摆放方式、无机盐浓度以及有机添加物对蝴蝶兰丛生芽的增殖均有显著影响,单芽切除生长点后横插(PSP-3)处理的单芽平均诱导芽数最高,但死亡率也达到23.3％,双芽切除生长点后横插(PSP-6)在控制死亡率的同时能获得较高的诱导芽数；在1/3 MS～1/2 MS之间的无机盐浓度比较适合于蝴蝶兰丛生芽增殖,且种苗长势健壮；15％ (v/v)椰汁最适于蝴蝶兰丛生芽增殖,150 g·L-1的马铃薯或150 g·L-1香蕉泥也可作为低成本有机添加物.