FIBERTEC 2010半自动纤维分析仪测定饲料中纤维素、半纤维素、木质素的方法研究

摘要

范氏洗涤纤维分析法(Van Soest法)在国际上已广泛使用,但采用传统的操作方法测定结果的精密度不理想.采用传统操作方法和FIBERTEC 2010半自动纤维分析仪分别测定饲料中纤维素、半纤维素、木质素的含量.结果表明采用Fibertec 2010半自动纤维分析仪法测定精密度高,RSD＜4％,平行样的重现性优于传统操作法,并发现添加硅藻土有助于提高试验结果的重现性.同时,对传统操作法所参考文献中的计算公式进行探讨,并提出修改和补充意见.%Van Soest method is commonly applied for fiber analysis worldwide,even though its measurement is known to be imprecise.Using an FIBERTEC 2010 Semi-automatic Fiber Analyzer,we determined the contents of cellulose,hemicellulose and lignin in feed to compare with the results obtained by the Van Soest method.The new method showed a variation coefficient of RSD less than 4％ with a significantly improved degree of precision.This article presents the modified methodology as well as a formula for result calculation.