为高值化开发利用鱼类加工副产物鱼鳞,以鱼鳞Ⅰ型胶原为原料,结合壳聚糖进行交联反应,通过正交实验优化制备工艺,获得具有蜂窝状多孔三维立体结构的鱼鳞胶原复合止血海绵.采用小鼠股动脉切面止血试验、豚鼠背部破损皮肤和健康皮肤的单次接触试验、小鼠肝脏吸附试验等对鱼鳞胶原复合止血海绵的止血特性、抗敏性能及生物相容性进行系统的比较研究.结果表明,鱼鳞胶原复合止血海绵最佳制备工艺为:胶原与壳聚糖配比7∶3,制备液总浓度1.4％,交联剂添加量0.015％,止血时间仅为(68±9)s；对豚鼠背部皮肤均无致敏性、无刺激性,安全性强；止血海绵经8周后可被小鼠的破损肝脏吸收.综上,鱼鳞胶原复合止血海绵具有良好的止血效果、安全性能和生物相容性.%For high-valued development and utilization of fish scale,an important fish-processing byproduct,after optimizing preparation technology through orthogonal experiments,fish scale collagen composite hemostatic sponge which possessed three-dimensional porous honeycomb structure was obtained by means of cross-linking reaction which combined type Ⅰ fish scale collagen with chitosan.Then a series of systematic comparative studies about the hemostatic and antiallergic property and biocompatibility of the composite sponge were carried out through hemostasis test on mouse femoral artery incision,single-contact test on both broken and healthy dorsal skin of guinea pig,and adsorption test on mouse liver.The results showed that the optimum preparation process of the fish scale collagen composite hemostatic sponge was as following:the ratio of collagen to chitosan was 7 ∶ 3,the preparing total concentration was 1.4 ％,the amount of crosslinking agent was 0.015 ％ and the hemostatic time was (68± 9) s.The sponge possessed strong security since there was no sensitization and irritation to the dorsal skin of guinea pig,and it could be assimilated by mouse liver after eight weeks.Therefore,the fish scale collagen composite hemostatic sponge possessed remarkable hemostatic effect,safety performance and biocompatibility.
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