BACKGROUND: Collagen has good hemostatic effect, but the single collagen hemostatic material has no ideal hemostatic efficiency due to limited water absorption capacity and poor blood concentration effect. OBJECTIVE:To observe the hemostatic effect of composite collagen sponge in vivo and in vitro. METHODS: Collagen, sponge and calcium chloride were used as raw materials to prepare composite collagen sponge. (1) Cell toxicity test:Mouse L929 cells were cultured using composite collagen sponge extract, phenol solution and cell culture medium for 2, 4, 7 days. Then, cell proliferation was detected by MTT to evaluate cell toxicity. (2) The relationship between the material and the whole blood: The relationship between the materials and the whole blood was observed by scanning electron microscope among composite collagen sponge, imported hemostatic materials and domestic hemostatic materials,following dripping the rabbit's anticoagulant blood onto the material surface.(3)In vitro coagulation activity:Blood Clotting Index was used to detect the effect of coagulation among composite collagen sponge,imported hemostatic materials and domestic hemostatic materials, following dripping the rabbit's anticoagulant blood onto the material surface. (4) Liver leaves from 18 rabbits were drilled, and then randomly divided into three groups covered with composite collagen sponge, imported hemostatic materials and domestic hemostatic materials, respectively. Bleeding time and bleeding volume were measured. RESULTS AND CONCLUSION: (1) The cytotoxicity of composite collagen sponge was qualified as grade 1. (2) Compared with imported and domestic hemostatic materials, composite collagen sponge was conducive to blood water absorption but also to the further network hemoglobin and platelet clot formation, and the pores alternately extended to the interior to form the alternating complex sponge. (3) The blood clotting index of composite collagen sponge was significantly lower than that of imported and domestic hemostatic materials (P < 0.05). (4) The bleeding time and bleeding volume of composite collagen sponge were significantly lower than those of imported and domestic hemostatic materials (P < 0.05). Overall, the composite collagen sponge as a hemostatic material has good hemostatic effect and biocompatibility.%背景:胶原材料具有良好的止血效果,但是单一胶原止血材料的吸水能力有限,血液浓缩效果不佳,所以止血效率并不理想.目的:观察复合胶原海绵止血材料的体内外止血效果.方法:以胶原、透明质酸和氯化钙为原料、碳化二酰亚胺为交联剂,制备复合胶原海绵止血材料.①细胞毒性实验:分别以复合胶原海绵止血材料浸提液、苯酚溶液及细胞培养液培养L929小鼠成纤维细胞,培养第2,4,7天,MTT 法检测细胞增殖,判定材料毒性;②材料与全血的关系:将兔抗凝血分别滴于复合胶原海绵止血材料、进口止血材料及国产止血材料表面,扫描电镜观察材料与全血的关系;③体外凝血活性:将兔抗凝血分别滴于复合胶原海绵止血材料、进口止血材料及国产止血材料表面,采用凝血指数检测凝血效果;④体内止血效果:钻透18只兔肝叶,随机分3组,分别以复合胶原海绵止血材料、进口止血材料及国产止血材料止血,检测出血时间及出血量.结果与结论:①复合胶原海绵材料的细胞毒性为1级,合格;②与进口和国产止血材料相比较,复合胶原海绵止血材料既有利于血液中水分继续向纵深吸收又能同时网络血红蛋白、血小板等形成凝血块,还可看到孔隙交替向内部延伸,形成交替复杂的海绵状;③复合胶原海绵止血材料的凝血指数显著低于进口、国产止血材料(P < 0.05);④复合胶原海绵止血材料组的出血时间及出血量明显低于进口止血材料组、国产止血材料组(P < 0.05);⑤结果表明,复合胶原海绵止血材料具有的良好止血效果及生物相容性.
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