禽坦布苏病毒包膜蛋白E的基因特征

摘要

The envelop protein fragments of avian Tembusu virus were amplified by RT-PCR from the identified avian Tembusu virus isolated in Fujian ,and cloned to the T-vector for sequenceing and bioinformatics analysis .The results revealed that the avian Tembusu virus envelop gene contained 1 053 nucleotides ,coding an open reading frame (ORF) with 501 amino acids .The molecular weights ,theoretical isoelectric point and the instability index of avian Tembusu virus envelop protein were 54.217 ku ,6.89 and 25.48 ,respectively .The topology predicted by N-best using the TMHMM2.0 is o442-464i471-489o .The sequenced envelop protein shared the highest homogeneity with Peking duck-origin avian Tembusu virus strain (GenBank accession number is JF459991) ,which was 99.8% , while shared with common sheldduck-origin avian Tembusu virus strain (GenBank accession number is JQ928189) 98.2% homogeneity .The results demonstrated that avian Tembusu virus had no host specificity.%为明确禽坦布苏病毒包膜蛋白E的基因特征，运用RT-PCR从已经分离鉴定的禽坦布苏病毒中扩增出其包膜蛋白E全基因，并进行克隆测序和分析。结果表明，禽坦布苏病毒包膜蛋白E全长为1503bp，编码501个氨基酸。所编码的包膜蛋白E大小为54.217ku，理论等电点为6.89，不稳定系数为25.48，属于稳定蛋白质类。利用TMHMM2.0进行跨膜区分析发现，该蛋白的拓扑结构为o442-464i471-489o。本试验株包膜蛋白E和北京鸭源坦布苏病毒（GenBank号：JF459991）同源性最高，达99.8％，和雌麻鸭源坦布苏病毒（GenBank号：JQ928189）核苷酸同源性最低，为98.2％，不同来源（鸡源、鸭源、鹅源、鸽源和蚊子源）坦布苏病毒包膜蛋白E同源性均较高，没有表现出宿主特异性。