大杯蕈菇柄多糖微波提取工艺优化

摘要

Microwave was applied for the extraction of polysaccharides from the stems of Clitocybe maxima .The parameters studied in the single-factor orthogonal experiment included material to liquid ratio ,extraction time ,and microwave power .The optimum conditions obtained were :the material to liquid ratio ,1∶ 25;extract duration , 8min ;and ,microwave power ,540 W .Among them ,microwave power exerted the greatest effect ,while extraction time the least ,on the polysaccharide extraction rate .Compared to the conventional hot water extraction , the new method had a 57.7% higher yield and 30.8% more polysaccharides .%采用微波辅助法提取大杯蕈菇柄多糖。通过考察料液比、微波提取时间以及微波功率3个因素，设计正交试验，得出微波辅助提取大杯蕈菇柄多糖的优化工艺条件为：料液比1∶25、微波时间8 min、微波功率540 W ，影响提取效果的因素效应主次顺序为微波功率＞料液比＞微波时间。与传统热水浸提方法相比较，微波辅助法的大杯蕈菇柄多糖提取率和纯度分别提高了57.7％和30.8％。微波辅助法提取可以显著提高大杯蕈菇柄多糖提取效率。