铁皮石斛白绢病菌的分离鉴定与抑菌药剂筛选

摘要

通过对分离到的铁皮石斛白绢病菌进行形态特征观察、致病性测定及 rDNA-ITS 的同源性分析，确定侵染铁皮石斛的病原菌为 Sclerotium rolfsii Sacc ．（有性态为 Athelia rolfsii ），与罗氏阿太菌（Athelia rolfsii ，登录号：HQ420816．1）同源性达到100％。选择5种药剂多利维生、80％乙蒜素、三唑酮、百菌清、奥力克-霉止，采用菌丝生长速率法，对白绢病菌进行室内抑菌测定。经室内抑菌试验及方差分析结果表明，多利维生与80％乙蒜素抑制率均可达87％以上，与对照相比达极显著水平。%The pathogen that causedSclerotialblight in Dendrobium officinaleKimura et Migo was identified to beSclerotiumrolfsii Sacc.by its morphological characteristics,as well as through the pathogenicity test and sequence homology analysis of its rDNA-ITS.The ITS sequence of the funguswas 100% identical with that of Atheliarolfsii (GenBank ID:HQ420816.1 ).Subsequently,a toxicity test on 5 fungicides for controlling S . rolfsii was conducted in the laboratory by using mycelium growth rate method and variance analysis.The results showed thatpolyversum and 80% ethylicin hada significant effect on controlling the pathogen with aninhibitory rate exceeding 87%,which reached an extremely significant level.