以杏鲍菇菌糠为培养基,发酵木霉(Trichoderma harzianum)Th3,获得菌糠木霉发酵物.将发酵物按照5个不同添加量掺入温室土壤中,盆栽不结球白菜Brassica cam pestris,研究发酵物的不同添加量对土壤pH、土壤蔗糖酶、土壤脲酶、不结球白菜根际木霉定殖数量、不结球白菜发芽率、出苗率、总生物量、叶绿素含量的影响.结果表明:当菌糠木霉发酵物与温室土壤比例为1∶39时,不结球白菜种子的出苗率明显提高,最高达到83.93％,不结球白菜叶片的叶绿素含量增加了51.23％,不结球白菜的总生物量提高了27.94％;土壤pH值提高至6.97,土壤蔗糖酶和脲酶活性得到提高,最高分别达到8.80、2.21 mg·g-1·d-1,木霉在不结球白菜根际的定殖数量明显提高,说明菌糠木霉发酵物具有很好的土壤改良效果,并能提高不结球白菜产量.%Trichoderma harzianum Th3 was inoculated on the spentsubstratefrom Pleurotus eryngii cultivation for fermentation.The resulting material wassubsequently mixed with the greenhouse soil at 5levels to evaluate their effects on the soil through observations on the growth of Brassica campestris planted on them.The pH,invertaseand urease in the soil mixture,the Th3countinrhizosphere,as well as the germination,emergence,total biomass,and chlorophyll content of the vegetable were determined.The results showed that the ratio of Th3 preparation to soil at 1 ∶ 39 resulted in significant increases onthe seedling emergence (83.93％ at maximum),the leaf chlorophyll content by 51.23％,thetotal biomass by 27.94％,the soil pH to 6.97,the invertase activity to 8.80 mg · g-1-d,the urease activity at 2.21 mg · g-1-d,and the enhancement of Th3 count in the rhizosphere.It appeared that addition of theTh3 preparation using the spent substrate from mushroom cultivation could improve the soil quality and yield of B.campestris.
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