短喙型鹅细小病毒感染雏鸭的排毒规律研究

摘要

本研究以短喙型鹅细小病毒(SBDS-GPV,M15株)口服感染2日龄健康易感半番鸭,采集感染后(PI)3、5、7、10、12、14、21 d的口咽和泄殖腔棉拭子,应用荧光定量PCR (qPCR)、病毒分离(VI)结合免疫荧光法(IFA)测定雏鸭感染后的排毒情况.结果显示:qPCR法检测SBDS GPV感染雏鸭的口咽和泄殖腔排毒时间分别为PI 3～10 d和PI 3～14 d;病毒分离结合IFA检测SBDS-GPV感染雏鸭的泄殖腔排毒时间为PI5～10 d.研究结果在国内外首次明确了雏鸭感染SBDS-GPV后可经口咽和泄殖腔向外界排毒,且泄殖腔排毒水平高于口咽.%Two-day-old mule ducklings were orally inoculated with short beak and dwarfism syndrome goose parvovirus (SBDS-GPV,strain M15) for this study.Swab samples from oropharynx and cloaca of the infected birds were collected after 3,5,7,10,12,14,and 21 days post infection (dPI) for the virus detection by quantitative real time PCR (qPCR),virus isolation (VI),and immunofluorescence (IFA).As determined by qPCR,the excretion of SBDS-GPV through oropharynx occurred in 3-10 dPI,and cloaca,in 3-14 dPI.On the other hand,the VI and IFA tests indicated that the cloacal excretion happened in 5-10 dPI.The results,for the first time,demonstrated that SBDS-GPV could pass through both oropharynx and cloaca of a diseased duckling,and that the greater extent of the excretion took place via cloaca.