为了揭示高 pH 对河蟹体色的潜在影响，探讨了高 pH 胁迫下河蟹虾青素和抗氧化能力的变化。将河蟹饲养于6个规格为18 m 2的水泥池中，养殖用水为沙滤河水（pH 7．5～8．0）。将其中三个水泥池的水用1 mol／L NaOH 调 pH 至9．0～9．5。分别于 pH 调整后的2、24、48、96、192 h 时采集河蟹血淋巴和头胸甲，分析头胸甲中虾青素含量、血清超氧化物歧化酶（SOD）活性、过氧化氢酶（CAT）活性和总抗氧化力（T－AOC）。结果表明：pH 胁迫后96 h 内河蟹头胸甲中虾青素含量显著下降（P ＜0．05），96 h 后虾青素含量趋于稳定；血清 SOD、 CAT、T－AOC 呈现先上升后下降的趋势， SOD 和 CAT 均在胁迫48 h 后与对照组差异不显著， T－AOC 在胁迫192 h 时与对照组差异不显著。实验结果表明高 pH 胁迫会导致河蟹氧化应激，从而使河蟹体色变差。%The lush aquatic plants grown in aquaculture ponds of Eriocheir sinensis cause high pH of pond water.In order to reveal the potential impact of the high pH on crab color , this study explored the response of crab astaxanthin content and antioxidant capacity to high pH.Crabs were reared in six cement pools(18 m 2 in size), and river water (pH7.5 ~8.0) was used after filtering by sand.The water pH of three cement pools was modulated to 9.0 to 9.5using 1 mol/L NaOH. Hemolymph and carapace of crabs were sampled at 2, 24, 48, 96, 192 h respectively after pH modulation.Astaxanthin content of carapace and the activity of serum superoxide dismutase (SOD), catalase (CAT) activity and total antioxidant capacity (T-AOC) were determined.The results showed that pH stress made the astaxanthin content of crab carapace de -crease significantly during 96 h (P <0.05), however, no further significant decrease was observed after 96 h.The SOD, CAT and T-AOC activity of serum present the trend of rising firstly and declining afterward .No significant decrease was ob-served in SOD and CAT after 48 h of stress, and no significant difference in T-AOC was detected after 192 h of stress com-pare with control group (P >0.05).These results indicated that high pH contributed poor color by arousing oxidative stress of crabs.
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