不同种质杜仲叶中多酚和黄酮含量差异性分析

摘要

[Objective]To compare the content difference of polyphenol and flavonoid in Eucommia ulmoides leav-es,so as to evaluate and utilize E.ulmoides germplasm reasonably.[Method]HPLC and spectrophotometric meth-ods were used to measure the contents of polyphenol,total flavonoid,isoquercitrin and quercetin of 105 E.ulmoides germplasm.[Result]The mean content of quercetin was 0.33 mg·g -1 with the highest variation coefficient of 42.42%,while the mean content of total flavonoid was 15.92 mg·g -1 with the lowest variation coefficient of 19.35%;the mean content of isoquercitrin,total polyphenol was 3.37 mg·g -1 and 42.74 mg·g -1 ,respectively, with the variation coefficient of 34.42% and 23.72%.No significant correlation was found in the contents of these four components between male and female plant leaves.There was highly significant difference in the four compo-nents among different regions (P <0.01).Hebei was the region where the mean contents of isoquercitrin,total fla-vonoid,total polyphenol were the highest contrast with the other regions.The results of correlation analysis showed that there existed significant positive correlation among the isoquercitrin,total flavonoid and total polyphenol whereas no significant correlation between quercetin and total polyphenol.E.ulmoides germplasm resources could be divided into four groups according to the content difference of the four components,in which the contents of flavonoid com-pounds and polyphenol in group Ⅲ including 13 materials were higher than that of the other groups.[Conclusion] The contents of polyphenol and flavonoid in leaves of E.ulmoides were high and abundant diversity and variation were found in different germplasms,which showed great potential for selection and improvement.Thus,it could provide basis materials for breeding Eucommia ulmoides germplasm.%[目的]比较不同种质杜仲叶中多酚及黄酮含量的差异性，合理评价与利用杜仲种质资源。[方法]利用高效液相色谱法和紫外分光光度计法对105份杜仲种质叶中多酚、总黄酮、异槲皮苷及槲皮素的含量进行测定。[结果]表明：种质叶中槲皮素含量平均为0．33 mg·g －1，变异系数最大，为42．42％；总黄酮含量平均为15．92 mg·g －1，变异系数最小，为19．35％；异槲皮苷、多酚含量平均值分别为3．37、42．74 mg· g －1，变异系数分别为34．42％、23．72％。杜仲雌株和雄株叶中的多酚、总黄酮、异槲皮苷及槲皮素含量差异不著性。多酚及黄酮类物质在不同来源间均差异极显著（P ＜0．01），其中，河北地区杜仲叶片中的总黄酮、异槲皮苷和槲皮素平均含量均最高。相关性分析发现：多酚、总黄酮、异槲皮苷含量彼此间均呈显著或极显著正相关，而槲皮素含量与多酚含量间无显著相关性。综合评价4种成分含量高低，可将杜仲种质资源分为4 大类群，其中，类群Ⅲ（包括13份材料）的多酚和黄酮类活性成分含量均高于其他类群。[结论]杜仲种质叶片中多酚和总黄酮含量较高，且表现出丰富的多样性，有很大的选择和改良潜力，可为叶用杜仲资源的选育提供基础原材料。