DNS法测定红松松塔多糖含量的研究

摘要

为建立红松松塔多糖含量测定方法。应用DNS法分别测定还原糖及水解后总糖的含量，计算得出多糖含量。结果表明，以葡萄糖为对照品，此方法在20～67mg／L浓度范围内线性关系良好，回归方程A＝18．7C－0．2232（R2＝0．9993），平均加样回收率99．47％，RSD＝1．68％。此法稳定性、重现性、精密度等均在规定范围内，过程简单，操作简便，可快速得出结果。%Objective To establish a method for the determination of polysaccharides in Pinus ko‐raiensis corns .Methods Determine the content of reducing sugar and total sugar by DNS Meth‐od .Results Contrast to glucose ,the linear range was 20‐67mg/L (R 2 =0 .999 3) ,the average re‐covery was 99 .47% ,RSD=1 .68% .Conclusion The method is sensitive ,steady with good repeat‐ability .