Methods Development Toward the Measurement of Polyaromatic Hydrocarbon-DNAAdducts by Mass Spectrometry

摘要

Advances in sensitivity have been achieved for two modes of mass spectrometryapplied to standards related to DNA adducts; gas chromatography with electron-capture negative ion mass spectrometry, and fast-atom-bombardment mass spectrometry. These advances involve both sample preparation and instrument conditions. For sample preparation, three new chemical techniques, each targeted toward a class of PAH-DNA adducts, show promise for measuring DNA adducts with increased sensitivity or scope by mass spectrometry; superoxide oxidation, hydrazinolysis, and hydrogenation. Most attention was given in this project to the superoxide oxidation technique, which was brought to the femtomole level for the measurement of a standard of benzo(a)pyrene-r-7,t-8,c-10-tetrahydrotetrol as a model analyte. Improved instrument conditions increased the sensitivity for measuring polyaromatic hydrocarbon-DNA adducts by fast-atom-bombardment mass spectrometry.