一种焚烧骨骼的DNA提取方法

摘要

本文介绍了一种针对烧骨的DNA有效提取方法。对烧骨进行研磨制成骨粉，对骨粉进行大体系脱钙和裂解，采用Amicon® Ultra-15离心过滤器对获得的脱钙、裂解上清液进行浓缩，提高DNA的回收率，使用硅胶膜法对浓缩后的DNA溶液进行纯化，并经复合STR扩增检验。使用上述方法成功完成两起严重焚烧骨骼案件的检验，确定了受害人身源。%ABSTRACT:This paper introduces a new DNA extraction approach for burned skeletal remains. The burned bones were grinded into powder, and then decalcified and digested in a large volume. Amicon® Ultra-15 centrifugal filter device was adopted to concentrate the supernatant after decalciifcation and digestion in order to increase the DNA recovery efifciency. Then the concentrated DNA supernatant was puriifed using silica gel membrane method. Autosomal STR genotyping was performed with commercial STR typing kits. Using this method, we have successfully identiifed the severely burned bones in two real cases, providing the key evidences for solving these cases.